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dc.contributor.author
Fink, Michel
dc.contributor.author
Thompson, Jeffrey S.
dc.contributor.author
Thoma, Fritz
dc.date.accessioned
2018-09-20T12:08:54Z
dc.date.available
2017-06-09T17:14:38Z
dc.date.available
2018-09-20T12:08:54Z
dc.date.issued
2011-10-28
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0026210
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/41260
dc.identifier.doi
10.3929/ethz-b-000041260
dc.description.abstract
Histone H3 mutations in residues that cluster in a discrete region on the nucleosome surface around lysine 79 of H3 affect H3-K79 methylation, impair transcriptional silencing in subtelomeric chromatin, and reveal distinct contributions of histone H3 to various DNA-damage response and repair pathways. These residues might act by recruitment of silencing and DNA-damage response factors. Alternatively, their location on the nucleosome surface suggests a possible involvement in nucleosome positioning, stability and nucleosome interactions. Here, we show that the yeast H3 mutants hht2-T80A, hht2-K79E, hht2-L70S, and hht2-E73D show normal nucleosome positioning and stability in minichromosomes. However, loss of silencing in a subtelomeric URA3 gene correlates with a shift of the promoter nucleosome, while nucleosome positions and stability in the coding region are maintained. Moreover, the H3 mutants show normal repair of UV lesions by photolyase and nucleotide excision repair in minichromosomes and slightly enhanced repair in the subtelomeric region. Thus, these results support a role of those residues in the recruitment of silencing proteins and argue against a general role in nucleosome organization.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Contributions of Histone H3 Nucleosome Core Surface Mutations to Chromatin Structures, Silencing and DNA Repair
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
6
en_US
ethz.journal.issue
10
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e26210
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
006206116
ethz.publication.place
Lawrence, KS, USA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
08783 - Thoma, Fritz (Tit.-Prof.)
en_US
ethz.leitzahl.certified
08783 - Thoma, Fritz (Tit.-Prof.)
ethz.date.deposited
2017-06-09T17:14:54Z
ethz.source
ECIT
ethz.identifier.importid
imp59364ea4c031b86652
ethz.ecitpid
pub:68911
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-31T15:23:27Z
ethz.rosetta.lastUpdated
2018-09-20T12:08:56Z
ethz.rosetta.versionExported
true
ethz.COinS
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