Show simple item record

dc.contributor.author
Schuetz, Robert
dc.contributor.author
Kuepfer, Lars
dc.contributor.author
Sauer, Uwe
dc.date.accessioned
2018-06-07T15:07:03Z
dc.date.available
2017-06-08T16:29:32Z
dc.date.available
2018-06-07T15:07:03Z
dc.date.issued
2007-01
dc.identifier.other
10.1038/msb4100162
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/4128
dc.identifier.doi
10.3929/ethz-b-000004128
dc.description.abstract
To which extent can optimality principles describe the operation of metabolic networks? By explicitly considering experimental errors and in silico alternate optima in flux balance analysis, we systematically evaluate the capacity of 11 objective functions combined with eight adjustable constraints to predict 13C‐determined in vivo fluxes in Escherichia coli under six environmental conditions. While no single objective describes the flux states under all conditions, we identified two sets of objectives for biologically meaningful predictions without the need for further, potentially artificial constraints. Unlimited growth on glucose in oxygen or nitrate respiring batch cultures is best described by nonlinear maximization of the ATP yield per flux unit. Under nutrient scarcity in continuous cultures, in contrast, linear maximization of the overall ATP or biomass yields achieved the highest predictive accuracy. Since these particular objectives predict the system behavior without preconditioning of the network structure, the identified optimality principles reflect, to some extent, the evolutionary selection of metabolic network regulation that realizes the various flux states.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publ. Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject
13C-flux
en_US
dc.subject
Evolution
en_US
dc.subject
Flux balance analysis
en_US
dc.subject
Metabolic network
en_US
dc.subject
Network optimality
en_US
dc.title
Systematic evaluation of objective functions for predicting intracellular fluxes in Escherichia coli
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported
dc.date.published
2007-07-10
ethz.journal.title
Molecular Systems Biology
ethz.journal.volume
3
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
119
en_US
ethz.size
15 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
004931271
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03710 - Hafen, Ernst / Hafen, Ernst
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03710 - Hafen, Ernst / Hafen, Ernst
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
ethz.date.deposited
2017-06-08T16:29:59Z
ethz.source
ECIT
ethz.identifier.importid
imp59364b738c1fc16130
ethz.ecitpid
pub:14236
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-17T07:41:51Z
ethz.rosetta.lastUpdated
2018-11-07T11:30:29Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Systematic%20evaluation%20of%20objective%20functions%20for%20predicting%20intracellular%20fluxes%20in%20Escherichia%20coli&rft.jtitle=Molecular%20Systems%20Biology&rft.date=2007-01&rft.volume=3&rft.issue=1&rft.spage=119&rft.au=Schuetz,%20Robert&Kuepfer,%20Lars&Sauer,%20Uwe&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record