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dc.contributor.author
Richter, S. Helene
dc.contributor.author
Garner, Joseph P.
dc.contributor.author
Zipser, Benjamin
dc.contributor.author
Lewejohann, Lars
dc.contributor.author
Sachser, Norbert
dc.contributor.author
Touma, Chadi
dc.contributor.author
Schindler, Britta
dc.contributor.author
Chourbaji, Sabine
dc.contributor.author
Brandwein, Christiane
dc.contributor.author
Gass, Peter
dc.contributor.author
Stipdonk, Niek van
dc.contributor.author
Harst, Johanneke van der
dc.contributor.author
Spruijt, Berry
dc.contributor.author
Võikar, Vootele
dc.contributor.author
Wolfer, David Paul
dc.contributor.author
Würbel, Hanno
dc.date.accessioned
2018-09-20T12:01:23Z
dc.date.available
2017-06-09T17:20:15Z
dc.date.available
2018-09-20T12:01:23Z
dc.date.issued
2011-01-31
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0016461
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/41563
dc.identifier.doi
10.3929/ethz-b-000041563
dc.description.abstract
In animal experiments, animals, husbandry and test procedures are traditionally standardized to maximize test sensitivity and minimize animal use, assuming that this will also guarantee reproducibility. However, by reducing within-experiment variation, standardization may limit inference to the specific experimental conditions. Indeed, we have recently shown in mice that standardization may generate spurious results in behavioral tests, accounting for poor reproducibility, and that this can be avoided by population heterogenization through systematic variation of experimental conditions. Here, we examined whether a simple form of heterogenization effectively improves reproducibility of test results in a multi-laboratory situation. Each of six laboratories independently ordered 64 female mice of two inbred strains (C57BL/6NCrl, DBA/2NCrl) and examined them for strain differences in five commonly used behavioral tests under two different experimental designs. In the standardized design, experimental conditions were standardized as much as possible in each laboratory, while they were systematically varied with respect to the animals' test age and cage enrichment in the heterogenized design. Although heterogenization tended to improve reproducibility by increasing within-experiment variation relative to between-experiment variation, the effect was too weak to account for the large variation between laboratories. However, our findings confirm the potential of systematic heterogenization for improving reproducibility of animal experiments and highlight the need for effective and practicable heterogenization strategies.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Effect of Population Heterogenization on the Reproducibility of Mouse Behavior: A Multi-Laboratory Study
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
6
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e16461
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
006206116
ethz.publication.place
Lawrence, Kan.
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02535 - Institut für Bewegungswiss. und Sport / Institut of Human Movement Sc. and Sport::03727 - Wolfer, David P. / Wolfer, David P.
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02535 - Institut für Bewegungswiss. und Sport / Institut of Human Movement Sc. and Sport::03727 - Wolfer, David P. / Wolfer, David P.
ethz.date.deposited
2017-06-09T17:20:29Z
ethz.source
ECIT
ethz.identifier.importid
imp59364ea9723d741688
ethz.ecitpid
pub:69468
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-26T18:41:49Z
ethz.rosetta.lastUpdated
2022-03-28T21:21:02Z
ethz.rosetta.versionExported
true
ethz.COinS
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