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dc.contributor.author
Mariotti, Laura
dc.contributor.author
Wild, Sebastian
dc.contributor.author
Brunoldi, Giulia
dc.contributor.author
Piceni, Alessandra
dc.contributor.author
Ceppi, Ilaria
dc.contributor.author
Kummer, Sandra
dc.contributor.author
Lutz, Richard E.
dc.contributor.author
Cejka, Petr
dc.contributor.author
Gari, Kerstin
dc.date.accessioned
2020-07-08T09:35:11Z
dc.date.available
2020-06-29T06:31:40Z
dc.date.available
2020-07-08T09:35:11Z
dc.date.issued
2020
dc.identifier.issn
2399-3642
dc.identifier.other
10.1038/s42003-020-1048-4
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/423502
dc.identifier.doi
10.3929/ethz-b-000423502
dc.description.abstract
The nuclease/helicase DNA2 plays important roles in DNA replication, repair and processing of stalled replication forks. DNA2 contains an iron-sulphur (FeS) cluster, conserved in eukaryotes and in a related bacterial nuclease. FeS clusters in DNA maintenance proteins are required for structural integrity and/or act as redox-sensors. Here, we demonstrate that loss of the FeS cluster affects binding of human DNA2 to specific DNA substrates, likely through a conformational change that distorts the central DNA binding tunnel. Moreover, we show that the FeS cluster is required for DNA2’s nuclease, helicase and ATPase activities. Our data also establish that oxidation of DNA2 impairs DNA binding in vitro, an effect that is reversible upon reduction. Unexpectedly, though, this redox-regulation is independent of the presence of the FeS cluster. Together, our study establishes an important structural role for the FeS cluster in human DNA2 and discovers a redox-regulatory mechanism to control DNA binding.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
The iron–sulphur cluster in human DNA2 is required for all biochemical activities of DNA2
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2020-06-23
ethz.journal.title
Communications Biology
ethz.journal.volume
3
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Commun Biol
ethz.pages.start
322
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2020-06-29T06:31:45Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-07-08T09:35:21Z
ethz.rosetta.lastUpdated
2022-03-29T02:36:32Z
ethz.rosetta.versionExported
true
ethz.COinS
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