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dc.contributor.author
Femmer, Christian
dc.contributor.author
Bechtold, Matthias
dc.contributor.author
Panke, Sven
dc.date.accessioned
2020-07-10T15:29:52Z
dc.date.available
2020-06-30T03:17:11Z
dc.date.available
2020-07-10T15:29:52Z
dc.date.issued
2020
dc.identifier.issn
0006-3592
dc.identifier.issn
1097-0290
dc.identifier.other
10.1002/bit.27449
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/423676
dc.description.abstract
Enzymes are industrially applied under increasingly diverse environmental conditions that are dictated by the efforts to optimize overall process efficiency. Engineering the operational stability of biocatalysts to enhance their half‐lives under the desired process conditions is a widely applied strategy to reduce costs. Here, we present a simple method to enhance enzyme stability in the presence of monophasic aqueous/organic solvent mixtures based on the concept of strengthening the enzyme's surface hydrogen‐bond network by exchanging surface‐located amino acid residues for arginine. Suitable residues are identified from sequence comparisons with homologous enzymes from thermophilic organisms and combined using a shuffling approach to obtain an enzyme variant with increased stability in monophasic aqueous/organic solvent mixtures. With this approach, we increase the stability of the broad‐spectrum amino acid racemase of Pseudomonas putida DSM 3263 eightfold in mixtures with 40% methanol and sixfold in mixtures with 30% acetonitrile. (© 2020 Wiley Periodicals LLC).
en_US
dc.language.iso
en
en_US
dc.publisher
WILEY
en_US
dc.subject
Enzyme engineering
en_US
dc.subject
Integrated processes
en_US
dc.subject
Nonconventional media
en_US
dc.subject
Process intensification
en_US
dc.subject
Racemase engineering
en_US
dc.title
Semi-rational engineering of an amino acid racemase that is stabilized in aqueous/organic solvent mixtures
en_US
dc.type
Journal Article
dc.date.published
2020-06-03
ethz.journal.title
Biotechnology and Bioengineering
ethz.journal.abbreviated
Biotechnol. Bioeng.
ethz.identifier.wos
ethz.publication.place
New York , NY
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2020-06-30T03:17:17Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.exportRequired
true
ethz.COinS
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