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dc.contributor.author
Schulz, Martin
dc.contributor.author
Calabrese, Silvia
dc.contributor.author
Wurm, Holger
dc.contributor.author
Drossart, Dominik
dc.contributor.author
Stock, Karl
dc.contributor.author
Sobieraj, Anna M.
dc.contributor.author
Eichenseher, Fritz
dc.contributor.author
Loessner, Martin J.
dc.contributor.author
Schmelcher, Mathias
dc.contributor.author
Gerhardts, Anja
dc.contributor.author
Goetz, Ulrike
dc.contributor.author
Handel, Marina
dc.contributor.author
Serr, Annerose
dc.contributor.author
Haecker, Georg
dc.contributor.author
Li, Jia
dc.contributor.author
Specht, Mara
dc.contributor.author
Koch, Philip
dc.contributor.author
Meyer, Martin
dc.contributor.author
Tepper, Philipp
dc.contributor.author
Rother, Raimund
dc.contributor.author
Jehle, Michael
dc.contributor.author
Wadle, Simon
dc.contributor.author
Zengerle, Roland
dc.contributor.author
von Stetten, Felix
dc.contributor.author
Paust, Nils
dc.contributor.author
Borst, Nadine
dc.date.accessioned
2020-08-07T10:49:46Z
dc.date.available
2020-07-02T02:49:30Z
dc.date.available
2020-07-07T11:47:44Z
dc.date.available
2020-08-07T10:46:35Z
dc.date.available
2020-08-07T10:49:46Z
dc.date.issued
2020-06-22
dc.identifier.issn
1473-0197
dc.identifier.issn
1473-0189
dc.identifier.other
10.1039/d0lc00294a
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/424120
dc.identifier.doi
10.3929/ethz-b-000424120
dc.description.abstract
We present an automated point-of-care testing (POCT) system for rapid detection of species- and resistance markers in methicillin-resistant Staphylococcus aureus (MRSA) at the level of single cells, directly from nasal swab samples. Our novel system allows clear differentiation between MRSA, methicillin-sensitive S. aureus (MSSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS), which is not the case for currently used real-time quantitative PCR based systems. On top, the novel approach outcompetes the culture-based methods in terms of its short time-to-result (1 h vs. up to 60 h) and reduces manual labor. The walk-away test is fully automated on the centrifugal microfluidic LabDisk platform. The LabDisk cartridge comprises the unit operations swab-uptake, reagent pre-storage, distribution of the sample into 20 000 droplets, specific enzymatic lysis of Staphylococcus spp. and recombinase polymerase amplification (RPA) of species (vicK) – and resistance (mecA) -markers. LabDisk actuation, incubation and multi-channel fluorescence detection is demonstrated with a clinical isolate and spiked nasal swab samples down to a limit of detection (LOD) of 3 ± 0.3 CFU μl−1 for MRSA. The novel approach of the digital single cell detection is suggested to improve hospital admission screening, timely decision making, and goal-oriented antibiotic therapy. The implementation of a higher degree of multiplexing is required to translate the results into clinical practice. © 2020 The Royal Society of Chemistry.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Royal Society of Chemistry
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/3.0/
dc.title
Point-of-care testing system for digital single cell detection of MRSA directly from nasal swabs
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 3.0 Unported
dc.date.published
2020-06-22
ethz.journal.title
Lab on a Chip
ethz.journal.volume
20
en_US
ethz.journal.issue
14
en_US
ethz.journal.abbreviated
Lab chip
ethz.pages.start
2549
en_US
ethz.pages.end
2561
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Cambridge
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2020-07-02T02:49:35Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-08-07T10:46:50Z
ethz.rosetta.lastUpdated
2020-08-07T10:50:06Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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