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dc.contributor.author
Devers, Emanuel
dc.contributor.author
Brosnan, Christopher A.
dc.contributor.author
Sarazin, Alexis
dc.contributor.author
Albertini, Daniele
dc.contributor.author
Amsler, Andrea C.
dc.contributor.author
Brioudes, Florian
dc.contributor.author
Jullien, Pauline E.
dc.contributor.author
Lim, PeiQi
dc.contributor.author
Schott, Grégory
dc.contributor.author
Voinnet, Olivier
dc.date.accessioned
2020-07-13T05:34:10Z
dc.date.available
2020-07-08T07:12:05Z
dc.date.available
2020-07-13T05:33:15Z
dc.date.available
2020-07-13T05:34:10Z
dc.date.issued
2020-07
dc.identifier.issn
2055-026X
dc.identifier.issn
2055-0278
dc.identifier.other
10.1038/s41477-020-0687-2
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/425140
dc.description.abstract
In RNA interference (RNAi), the RNase III Dicer processes long double-stranded RNA (dsRNA) into short interfering RNA (siRNA), which, when loaded into ARGONAUTE (AGO) family proteins, execute gene silencing1. Remarkably, RNAi can act non-cell autonomously2,3: it is graft transmissible4,5,6,7, and plasmodesmata-associated proteins modulate its cell-to-cell spread8,9. Nonetheless, the molecular mechanisms involved remain ill defined, probably reflecting a disparity of experimental settings. Among other caveats, these almost invariably cause artificially enhanced movement via transitivity, whereby primary RNAi-target transcripts are converted into further dsRNA sources of secondary siRNA5,10,11. Whether siRNA mobility naturally requires transitivity and whether it entails the same or distinct signals for cell-to-cell versus long-distance movement remains unclear, as does the identity of the mobile signalling molecules themselves. Movement of long single-stranded RNA, dsRNA, free/AGO-bound secondary siRNA or primary siRNA have all been advocated12,13,14,15; however, an entity necessary and sufficient for all known manifestations of plant mobile RNAi remains to be ascertained. Here, we show that the same primary RNAi signal endows both vasculature-to-epidermis and long-distance silencing movement from three distinct RNAi sources. The mobile entities are AGO-free primary siRNA duplexes spreading length and sequence independently. However, their movement is accompanied by selective siRNA depletion reflecting the AGO repertoires of traversed cell types. Coupling movement with this AGO-mediated consumption process creates qualitatively distinct silencing territories, potentially enabling unlimited spatial gene regulation patterns well beyond those granted by mere gradients.
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.title
Movement and differential consumption of short interfering RNA duplexes underlie mobile RNA interference
en_US
dc.type
Journal Article
dc.date.published
2020-07-06
ethz.journal.title
Nature Plants
ethz.journal.volume
6
en_US
ethz.journal.issue
7
en_US
ethz.journal.abbreviated
Nat Plants
ethz.pages.start
789
en_US
ethz.pages.end
799
en_US
ethz.grant
High resolution and chemical genetic approaches to RNA silencing mechanisms
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03876 - Voinnet, Olivier / Voinnet, Olivier
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03876 - Voinnet, Olivier / Voinnet, Olivier
en_US
ethz.grant.agreementno
323071
ethz.grant.fundername
EC
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.program
FP7
ethz.date.deposited
2020-07-08T07:12:16Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2020-07-13T05:33:26Z
ethz.rosetta.lastUpdated
2021-02-15T15:25:12Z
ethz.rosetta.versionExported
true
ethz.COinS
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