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dc.contributor.author
Zarza, Xavier
dc.contributor.author
Van Wijk, Ringo
dc.contributor.author
Shabala, Lana
dc.contributor.author
Hunkeler, Anna
dc.contributor.author
Lefebvre, Matthew
dc.contributor.author
Rodriguez-Villalon, Antia
dc.contributor.author
Shabala, Sergey
dc.contributor.author
Tiburcio, Antonio F.
dc.contributor.author
Heilmann, Ingo
dc.contributor.author
Munnik, Teun
dc.date.accessioned
2020-10-30T15:51:26Z
dc.date.available
2020-07-16T14:52:23Z
dc.date.available
2020-07-20T11:49:20Z
dc.date.available
2020-10-30T15:51:26Z
dc.date.issued
2020-10
dc.identifier.issn
0960-7412
dc.identifier.issn
1365-313X
dc.identifier.other
10.1111/tpj.14932
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/426685
dc.identifier.doi
10.3929/ethz-b-000426685
dc.description.abstract
Polyamines, such as putrescine, spermidine and spermine (Spm), are low‐molecular‐weight polycationic molecules present in all living organisms. Despite their implication in plant cellular processes, little is known about their molecular mode of action. Here, we demonstrate that polyamines trigger a rapid increase in the regulatory membrane lipid, phosphatidylinositol‐4,5‐bisphosphate (PIP2), and that this increase is required for polyamine effects on K+ efflux in Arabidopsis roots. Using in vivo 32Pi‐labelling of Arabidopsis seedlings, low physiological (μM) concentrations of Spm were found to promote a rapid PIP2 increase in roots that was time‐ and dose‐dependent. Confocal imaging of a genetically encoded‐PIP2 biosensor revealed that this increase was triggered at the plasma membrane. Differential 32Pi‐labelling suggested that the increase in PIP2 was generated through activation of phosphatidylinositol‐4‐phosphate 5‐kinase (PIP5K) activity rather than inhibition of a phospholipase C or PIP2 5‐phosphatase activity. Systematic analysis of T‐DNA insertion mutants identified PIP5K7 and PIP5K9 as the main candidates involved in the Spm induced‐PIP2 response. Using non‐invasive microelectrode ion‐flux estimation (MIFE), we discovered that the Spm‐triggered K+‐efflux response was strongly reduced in pip5k7 pip5k9 seedlings. Together, our results provide biochemical‐ and genetic evidence for a physiological role of PIP2 in polyamine‐mediated signalling controlling K+ flux in plants.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Arabidopsis
en_US
dc.subject
Phosphoinositide signalling
en_US
dc.subject
Phosphatidylinositol 4,5-bisphosphate (PIP2)
en_US
dc.subject
Phosphatidylinositol 4-phosphate 5-kinase (PIP5K)
en_US
dc.subject
Phosphateidic acid (PA)
en_US
dc.subject
Phospholipids
en_US
dc.subject
Polyamines
en_US
dc.subject
K+ flux
en_US
dc.title
Lipid kinases PIP5K7 and PIP5K9 are required for polyamine‐triggered K+ efflux in Arabidopsis roots
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2020-07-15
ethz.journal.title
The Plant Journal
ethz.journal.volume
104
en_US
ethz.journal.issue
2
en_US
ethz.journal.abbreviated
Plant j.
ethz.pages.start
416
en_US
ethz.pages.end
432
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Phosphoinositides- Molecular analysis of new lipid regulators in plant phloem differentiation
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::09493 - Rodriguez-Villalon, Antia / Rodriguez-Villalon, Antia
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::09493 - Rodriguez-Villalon, Antia / Rodriguez-Villalon, Antia
en_US
ethz.grant.agreementno
160201
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.date.deposited
2020-07-16T14:52:34Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-10-30T15:51:35Z
ethz.rosetta.lastUpdated
2021-02-15T19:31:00Z
ethz.rosetta.versionExported
true
ethz.COinS
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