Quantification of Mechanical Forces and Physiological Processes Involved in Pollen Tube Growth Using Microfluidics and Microrobotics
- Book Chapter
Pollen tubes face many obstacles on their way to the ovule. They have to decide whether to navigate around cells or penetrate the cell wall and grow through it or even within it. Besides chemical sensing, which directs the pollen tubes on their path to the ovule, this involves mechanosensing to determine the optimal strategy in specific situations. Mechanical cues then need to be translated into physiological signals, which eventually lead to changes in the growth behavior of the pollen tube. To study these events, we have developed a system to directly quantify the forces involved in pollen tube navigation. We combined a lab-on-a-chip device with a microelectromechanical systems-based force sensor to mimic the pollen tube’s journey from stigma to ovary in vitro. A force-sensing plate creates a mechanical obstacle for the pollen tube to either circumvent or attempt to penetrate while measuring the involved forces in real time. The change of growth behavior and intracellular signaling activities can be observed with a fluorescence microscope. Show more
Book titlePollen and Pollen Tube Biology
Journal / seriesMethods in Molecular Biology
Pages / Article No.
SubjectPollen tube (PT); Growth; Force sensor; Perceptive force; Penetrative force; Lab-on-a-chip (LOC); Microelectromechanical system (MEMS); Calcium (Ca2+); Imaging; Fluorescence
Organisational unit03627 - Nelson, Bradley J. / Nelson, Bradley J.
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