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Date
2020-08Type
- Journal Article
Citations
Cited 47 times in
Web of Science
Cited 56 times in
Scopus
ETH Bibliography
yes
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Abstract
In response to pathogenic threats, naive T cells rapidly transition from a quiescent to an activated state, yet the underlying mechanisms are incompletely understood. Using a pulsed SILAC approach, we investigated the dynamics of mRNA translation kinetics and protein turnover in human naive and activated T cells. Our datasets uncovered that transcription factors maintaining T cell quiescence had constitutively high turnover, which facilitated their depletion following activation. Furthermore, naive T cells maintained a surprisingly large number of idling ribosomes as well as 242 repressed mRNA species and a reservoir of glycolytic enzymes. These components were rapidly engaged following stimulation, promoting an immediate translational and glycolytic switch to ramp up the T cell activation program. Our data elucidate new insights into how T cells maintain a prepared state to mount a rapid immune response, and provide a resource of protein turnover, absolute translation kinetics and protein synthesis rates in T cells (https://www.immunomics.ch). Show more
Publication status
publishedExternal links
Journal / series
Nature ImmunologyVolume
Pages / Article No.
Publisher
Nature Publishing GroupOrganisational unit
09604 - Sallusto, Federica / Sallusto, Federica
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Show all metadata
Citations
Cited 47 times in
Web of Science
Cited 56 times in
Scopus
ETH Bibliography
yes
Altmetrics