Zur Kurzanzeige

dc.contributor.author
You, Jae B.
dc.contributor.author
Lee, Byungjin
dc.contributor.author
Choi, Yunho
dc.contributor.author
Lee, Chang-Soo
dc.contributor.author
Peter, Matthias
dc.contributor.author
Im, Sung G.
dc.contributor.author
Lee, Sung Sik
dc.date.accessioned
2021-01-11T12:12:09Z
dc.date.available
2020-07-28T02:59:17Z
dc.date.available
2020-08-31T10:15:27Z
dc.date.available
2021-01-11T12:12:09Z
dc.date.issued
2020-07
dc.identifier.issn
0736-6205
dc.identifier.other
10.2144/btn-2020-0025
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/428717
dc.identifier.doi
10.3929/ethz-b-000428717
dc.description.abstract
Poly(dimethylsiloxane) (PDMS) is widely used as a microfluidics platform material; however, it absorbs various molecules, perturbing specific chemical concentrations in microfluidic channels. We present a simple solution to prevent adsorption into a PDMS microfluidic device. We used a vapor-phase-deposited nanoadhesive layer to seal PDMS microfluidic channels. Absorption of fluorescent molecules into PDMS was efficiently prevented in the nanolayer-treated PDMS device. Importantly, when cultured in a nanolayer-treated PDMS device, yeast cells exhibited the expected concentration-dependent response to a mating pheromone, including mating-specific morphological and gene expression changes, while yeast cultured in an untreated PDMS device did not properly respond to the pheromone. Our method greatly expands microfluidic applications that require precise control of molecule concentrations.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Future Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
device bonding
en_US
dc.subject
nanoadhesive layer
en_US
dc.subject
PDMS microfluidics
en_US
dc.subject
protein absorption
en_US
dc.subject
yeast mating
en_US
dc.title
Nanoadhesive layer to prevent protein absorption in a poly(dimethylsiloxane) microfluidic device
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.date.published
2020-05-06
ethz.journal.title
BioTechniques
ethz.journal.volume
69
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
BioTechniques
ethz.pages.start
47
en_US
ethz.pages.end
52
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03595 - Peter, Matthias / Peter, Matthias
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02891 - ScopeM / ScopeM
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03595 - Peter, Matthias / Peter, Matthias
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02891 - ScopeM / ScopeM
ethz.date.deposited
2020-07-28T02:59:23Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-08-31T10:15:38Z
ethz.rosetta.lastUpdated
2021-02-15T23:06:17Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Nanoadhesive%20layer%20to%20prevent%20protein%20absorption%20in%20a%20poly(dimethylsiloxane)%20microfluidic%20device&rft.jtitle=BioTechniques&rft.date=2020-07&rft.volume=69&rft.issue=1&rft.spage=47&rft.epage=52&rft.issn=0736-6205&rft.au=You,%20Jae%20B.&Lee,%20Byungjin&Choi,%20Yunho&Lee,%20Chang-Soo&Peter,%20Matthias&rft.genre=article&rft_id=info:doi/10.2144/btn-2020-0025&
 Printexemplar via ETH-Bibliothek suchen

Dateien zu diesem Eintrag

Thumbnail

Publikationstyp

Zur Kurzanzeige