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dc.contributor.author
Kappler, Katharina
dc.contributor.author
Lasanajak, Yi
dc.contributor.author
Smith, David F.
dc.contributor.author
Opitz, Lennart
dc.contributor.author
Hennet, Thierry
dc.date.accessioned
2020-08-10T08:56:10Z
dc.date.available
2020-08-06T14:25:21Z
dc.date.available
2020-08-10T08:56:10Z
dc.date.issued
2020
dc.identifier.issn
1664-302X
dc.identifier.other
10.3389/fmicb.2020.01553
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/430416
dc.identifier.doi
10.3929/ethz-b-000430416
dc.description.abstract
Inflammatory bowel disease is associated with intestinal dysbiosis and with elevated antibody production toward microbial epitopes. The underlying processes linking the gut microbiota with inflammation are still unclear. Considering the constant induction of antibodies by gut microbial glycans, the aim of this study was to address whether the repertoire of carbohydrate-specific antibodies is altered in Crohn’s disease or ulcerative colitis. IgG and IgM reactivities to oligosaccharides representative of mucosal glycans were tested in blood serum from 20 healthy control subjects, 17 ulcerative colitis patients, and 23 Crohn’s disease patients using glycan arrays. An increased IgG and IgM reactivity toward fucosylated oligosaccharides was detected in Crohn’s disease but not in ulcerative colitis. To address the antibody reactivity to the gut microbiota, IgG binding to members of a complex intestinal microbiota was measured and observed to be increased in sera of patients with Crohn’s disease. Based on the elevated reactivity to fucosylated oligosaccharides, gut bacteria were tested for recognition by the fucose-binding Aleuria aurantia lectin. Bacteroides stercoris was detected in IgG- and lectin-positive fractions and reactivity of A. aurantia lectin was demonstrated for additional Bacteroides species. IgG reactivity to these Bacteroides species was significantly increased in inflammatory bowel disease patients, indicating that the increased reactivity to fucosylated oligosaccharides detected in Crohn’s disease may be induced by fucose-carrying intestinal bacteria. Enhanced antibody response to fucosylated epitopes may have systemic effects by altering the binding of circulating antibodies to endogenous glycoproteins.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Research Foundation
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
IBD
en_US
dc.subject
Oligosaccharide
en_US
dc.subject
Array
en_US
dc.subject
Microbiota
en_US
dc.subject
Bacteroides
en_US
dc.subject
Fucose
en_US
dc.title
Increased Antibody Response to Fucosylated Oligosaccharides and Fucose-Carrying Bacteroides Species in Crohn’s Disease
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2020-07-14
ethz.journal.title
Frontiers in Microbiology
ethz.journal.volume
11
en_US
ethz.journal.abbreviated
Front Microbiol
ethz.pages.start
1553
en_US
ethz.size
15 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Lausanne
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
ethz.date.deposited
2020-08-06T14:25:34Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-08-10T08:56:31Z
ethz.rosetta.lastUpdated
2022-03-29T02:53:40Z
ethz.rosetta.versionExported
true
ethz.COinS
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