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dc.contributor.author
De Luca, Chiara
dc.contributor.author
Felletti, Simona
dc.contributor.author
Macis, Marco
dc.contributor.author
Cabri, Walter
dc.contributor.author
Lievore, Giulio
dc.contributor.author
Chenet, Tatiana
dc.contributor.author
Pasti, Luisa
dc.contributor.author
Morbidelli, Massimo
dc.contributor.author
Cavazzini, Alberto
dc.contributor.author
Catani, Martina
dc.contributor.author
Ricci, Antonio
dc.date.accessioned
2020-08-28T11:02:33Z
dc.date.available
2020-08-28T11:02:33Z
dc.date.issued
2020-04-12
dc.identifier.issn
0021-9673
dc.identifier.other
10.1016/j.chroma.2019.460789
dc.identifier.uri
http://hdl.handle.net/20.500.11850/437301
dc.description.abstract
The thermodynamic behavior of octreotide, a cyclic octapeptide with important pharmaceutical functions, has been simulated under reversed-phase gradient elution conditions. To this end, adsorption behavior was firstly investigated in isocratic conditions, under a variety of water/acetonitrile + 0.02% (v/v) trifluoroacetic acid (TFA) mixtures as mobile phase by using a Langmuir isotherm. Organic modifier was varied in the range between 23 and 28% (v/v). Adsorption isotherms were determined by means of the so-called Inverse Method (IM) with a minimum amount of peptide. The linear solvent strength (LSS) model was used to find the correlation between isotherm parameters and mobile phase composition. This study contributes to enlarge our knowledge on the chromatographic behavior under nonlinear gradient conditions of peptides. In particular, it focuses on a cyclic octapeptide. (C) 2019 Elsevier B.V. All rights reserved.
dc.publisher
ELSEVIER
dc.subject
Pharmaceutical peptides
dc.subject
Gradient elution
dc.subject
Adsorption isotherms
dc.subject
Reversed-phase liquid chromatography (RP-LC)
dc.subject
Inverse method
dc.subject
Preparative chromatography
dc.title
Modeling the nonlinear behavior of a bioactive peptide in reversed-phase gradient elution chromatography
dc.type
Journal Article
ethz.journal.title
Journal of Chromatography A
ethz.journal.volume
1616
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
AMSTERDAM
ethz.source
WOS
ethz.rosetta.exportRequired
true
ethz.COinS
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