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dc.contributor.author
Groninger, Olivier
dc.contributor.author
Hess, Samuel
dc.contributor.author
Mohn, Dirk
dc.contributor.author
Schneider, Elia
dc.contributor.author
Stark, Wendelin
dc.contributor.author
Marsmann, Sonja
dc.contributor.author
Wolint, Petra
dc.contributor.author
Calcagni, Maurizio
dc.contributor.author
Cinelli, Paolo
dc.contributor.author
Buschmann, Johanna
dc.date.accessioned
2020-08-28T11:02:34Z
dc.date.available
2020-08-28T11:02:34Z
dc.date.issued
2020-04-01
dc.identifier.issn
1422-0067
dc.identifier.other
10.3390/ijms21072627
dc.identifier.uri
http://hdl.handle.net/20.500.11850/437302
dc.description.abstract
The microenvironment of mesenchymal stem cells (MSCs) is responsible for the modulation in MSC commitment. Nanocomposites with an inorganic and an organic component have been investigated, and osteogenesis of MSCs has been attributed to inorganic phases such as calcium phosphate under several conditions. Here, electrospun meshes and two-dimensional films of poly(lactic-co-glycolic acid) (PLGA) or nanocomposites of PLGA and amorphous calcium phosphate nanoparticles (PLGA/aCaP) seeded with human adipose-derived stem cells (ASCs) were analyzed for the expression of selected marker genes. In a two-week in vitro experiment, osteogenic commitment was not found to be favored on PLGA/aCaP compared to pure PLGA. Analysis of the medium revealed a significant reduction of the Ca2+ concentration when incubated with PLGA/aCaP, caused by chemical precipitation of hydroxyapatite (HAp) on aCaP seeds of PLGA/aCaP. Upon offering a constant Ca2+ concentration, however, the previously observed anti-osteogenic effect was reversed: alkaline phosphatase, an early osteogenic marker gene, was upregulated on PLGA/aCaP compared to pristine PLGA. Hence, in addition to the cell-material interaction, the material-medium interaction was also important for the stem cell commitment here, affecting the cell-medium interaction. Complex in vitro models should therefore consider all factors, as coupled impacts might emerge.
dc.publisher
MDPI
dc.subject
adipose-derived stem cells
dc.subject
PLGA
dc.subject
amorphous calcium phosphate nanoparticles
dc.subject
calcium ions
dc.subject
gene expression
dc.title
Directing Stem Cell Commitment by Amorphous Calcium Phosphate Nanoparticles Incorporated in PLGA: Relevance of the Free Calcium Ion Concentration
dc.type
Journal Article
ethz.journal.title
International Journal of Molecular Sciences
ethz.journal.volume
21
ethz.journal.issue
7
ethz.journal.abbreviated
Int. j. mol. sci.
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
BASEL
ethz.source
WOS
ethz.rosetta.exportRequired
true
ethz.COinS
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