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dc.contributor.author
Prada-Luengo, Iñigo
dc.contributor.author
Moller, Henrik D.
dc.contributor.author
Henriksen, Rasmus A.
dc.contributor.author
Gao, Qian
dc.contributor.author
Eggert Larsen, Camilla
dc.contributor.author
Alizadeh, Sefa
dc.contributor.author
Maretty, Lasse
dc.contributor.author
Houseley, Jonathan
dc.contributor.author
Regenberg, Birgitte
dc.date.accessioned
2020-09-10T15:02:49Z
dc.date.available
2020-09-04T20:13:26Z
dc.date.available
2020-09-10T15:02:49Z
dc.date.issued
2020-08-20
dc.identifier.issn
1362-4962
dc.identifier.issn
0301-5610
dc.identifier.other
10.1093/nar/gkaa545
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/438600
dc.identifier.doi
10.3929/ethz-b-000438600
dc.description.abstract
© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. Circular DNA can arise from all parts of eukaryotic chromosomes. In yeast, circular ribosomal DNA (rDNA) accumulates dramatically as cells age, however little is known about the accumulation of other chromosome-derived circles or the contribution of such circles to genetic variation in aged cells. We profiled circular DNA in Saccharomyces cerevisiae populations sampled when young and after extensive aging. Young cells possessed highly diverse circular DNA populations but 94% of the circular DNA were lost after ∼15 divisions, whereas rDNA circles underwent massive accumulation to >95% of circular DNA. Circles present in both young and old cells were characterized by replication origins including circles from unique regions of the genome and repetitive regions: rDNA and telomeric Y' regions. We further observed that circles can have flexible inheritance patterns: [HXT6/7circle] normally segregates to mother cells but in low glucose is present in up to 50% of cells, the majority of which must have inherited this circle from their mother. Interestingly, [HXT6/7circle] cells are eventually replaced by cells carrying stable chromosomal HXT6 HXT6/7 HXT7 amplifications, suggesting circular DNAs are intermediates in chromosomal amplifications. In conclusion, the heterogeneity of circular DNA offers flexibility in adaptation, but this heterogeneity is remarkably diminished with age.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Replicative aging is associated with loss of genetic heterogeneity from extrachromosomal circular DNA in Saccharomyces cerevisiae
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2020-07-01
ethz.journal.title
Nucleic Acids Research
ethz.journal.volume
48
en_US
ethz.journal.issue
14
en_US
ethz.journal.abbreviated
Nucleic Acids Res.
ethz.pages.start
7883
en_US
ethz.pages.end
7898
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2020-09-04T20:14:01Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-09-10T15:03:01Z
ethz.rosetta.lastUpdated
2021-02-15T17:09:35Z
ethz.rosetta.versionExported
true
ethz.COinS
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