Delivery of myo‐Inositol Hexakisphosphate to the Cell Nucleus with a Proline‐Based Cell‐Penetrating Peptide
dc.contributor.author
Li, Mao
dc.contributor.author
Puschmann, Robert
dc.contributor.author
Herdlitschka, Andreas
dc.contributor.author
Fiedler, Dorothea
dc.contributor.author
Wennemers, Helma
dc.date.accessioned
2020-09-10T06:35:57Z
dc.date.available
2020-09-04T20:17:36Z
dc.date.available
2020-09-10T06:35:57Z
dc.date.issued
2020-09-01
dc.identifier.issn
1521-3757
dc.identifier.issn
0044-8249
dc.identifier.other
10.1002/ange.202006770
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/438628
dc.description.abstract
Inositol hexakisphosphate (InsP6) is a central member of the inositol phosphate messengers in eukaryotic cells. Tools to manipulate the level of InsP6, particularly with compartment selectivity, are needed to enable functional cellular studies. We present cationic octa-(4S)guanidiniumproline (Z8) for the delivery of InsP6 into the cell nucleus. CD spectroscopy, binding affinity, dynamic light scattering, and computational studies revealed that Z8 binds tightly to InsP6 and upon binding undergoes a conformational change from a PPII-helical structure to a structure that forms aggregates. The unique conformational features of the cationic oligoproline enable complex formation and cellular delivery of InsP6 with considerably greater efficacy than the flexible counterpart octaarginine. © 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley
en_US
dc.title
Delivery of myo‐Inositol Hexakisphosphate to the Cell Nucleus with a Proline‐Based Cell‐Penetrating Peptide
en_US
dc.type
Journal Article
dc.date.published
2020-06-18
ethz.journal.title
Angewandte Chemie
ethz.journal.volume
132
en_US
ethz.journal.issue
36
en_US
ethz.journal.abbreviated
Angew. Chem
ethz.pages.start
15716
en_US
ethz.pages.end
15719
en_US
ethz.identifier.scopus
ethz.publication.place
Weinheim
en_US
ethz.publication.status
published
en_US
ethz.relation.isVariantFormOf
20.500.11850/423459
ethz.date.deposited
2020-09-04T20:17:49Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2020-09-10T06:36:13Z
ethz.rosetta.lastUpdated
2020-09-10T06:36:13Z
ethz.rosetta.versionExported
true
ethz.COinS
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Journal Article [132298]