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dc.contributor.author
Dersch, Simon
dc.contributor.author
Mehl, Johanna
dc.contributor.author
Stuckenschneider, Lisa
dc.contributor.author
Mayer, Benjamin
dc.contributor.author
Roth, Julian
dc.contributor.author
Rohrbach, Alexander
dc.contributor.author
Graumann, Peter L.
dc.date.accessioned
2020-09-10T15:23:58Z
dc.date.available
2020-09-10T05:47:31Z
dc.date.available
2020-09-10T15:23:58Z
dc.date.issued
2020-08
dc.identifier.issn
1664-302X
dc.identifier.other
10.3389/fmicb.2020.01946
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/439491
dc.identifier.doi
10.3929/ethz-b-000439491
dc.description.abstract
The movement of filamentous, actin-like MreB and of enzymes synthesizing the bacterial cell wall has been proposed to be highly coordinated. We have investigated the motion of MreB and of RodA and PbpH cell wall synthesis enzymes at 500 ms and at 20 ms time scales, allowing us to compare the motion of entire MreB filaments as well as of single molecules with that of the two synthesis proteins. While all three proteins formed assemblies that move with very similar trajectory orientation and with similar velocities, their trajectory lengths differed considerably, with PbpH showing shortest and MreB longest trajectories. These experiments suggest different on/off rates for RodA and PbpH at the putative peptidoglycan-extending machinery (PGEM), and during interaction with MreB filaments. Single molecule tracking revealed distinct slow-moving and freely diffusing populations of PbpH and RodA, indicating that they change between free diffusion and slow motion, indicating a dynamic interaction with the PGEM complex. Dynamics of MreB molecules and the orientation and speed of filaments changed markedly after induction of salt stress, while there was little change for RodA and PbpH single molecule dynamics. During the stress adaptation phase, cells continued to grow and extended the cell wall, while MreB formed fewer and more static filaments. Our results show that cell wall synthesis during stress adaptation occurs in a mode involving adaptation of MreB dynamics, and indicate that Bacillus subtilis cell wall extension involves an interplay of enzymes with distinct binding kinetics to sites of active synthesis.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Media
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
cell shape maintenance
en_US
dc.subject
MreB cytoskeleton
en_US
dc.subject
Bacillus subtilis
en_US
dc.subject
RodA
en_US
dc.subject
single molecule dynamics
en_US
dc.title
Super-Resolution Microscopy and Single-Molecule Tracking Reveal Distinct Adaptive Dynamics of MreB and of Cell Wall-Synthesis Enzymes
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2020-08-20
ethz.journal.title
Frontiers in Microbiology
ethz.journal.volume
11
en_US
ethz.journal.abbreviated
Front Microbiol
ethz.pages.start
1946
en_US
ethz.size
19 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Lausanne
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
ethz.date.deposited
2020-09-10T05:47:36Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-09-10T15:24:12Z
ethz.rosetta.lastUpdated
2024-02-02T12:01:41Z
ethz.rosetta.versionExported
true
ethz.COinS
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