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dc.contributor.author
Chen, Zhenyue
dc.contributor.author
Zhou, Quanyu
dc.contributor.author
Rebling, Johannes
dc.contributor.author
Razansky, Daniel
dc.date.accessioned
2020-11-12T14:00:36Z
dc.date.available
2020-09-12T02:52:57Z
dc.date.available
2020-09-18T09:44:25Z
dc.date.available
2020-11-12T14:00:36Z
dc.date.issued
2020-11
dc.identifier.issn
1864-063X
dc.identifier.issn
1864-0648
dc.identifier.other
10.1002/jbio.202000198
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/440129
dc.description.abstract
The recently introduced large‐field multifocal illumination (LMI) fluorescence microscopy technique opened new possibilities for transcranial observations of mouse brain dynamics with a unique combination of capillary level resolution and centimeter‐scale field‐of‐view (FOV). Here we report on a new acceleration scheme for LMI based on raster scan of a lattice pattern combined with a parallel camera exposure scheme, which attains 200 Hz frame rate over 12 × 12 mm2 FOV with 7.5 μm spatial resolution. We demonstrate real‐time transcranial in vivo tracking of particles and imaging of microcirculation across the entire mouse cortex, thus corroborating the superb spatiotemporal resolution performance of LMI unattainable with other techniques. Potential applications include investigations into cerebrovascular function, cell tracking, as well as large‐scale functional neuroimaging. © 2020 Wiley-VCH GmbH.
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley
en_US
dc.subject
Brain dynamics
en_US
dc.subject
Cerebral vasculature
en_US
dc.subject
Fluorescence microscopy
en_US
dc.subject
In vivo tracking
en_US
dc.subject
Microcirculation
en_US
dc.subject
Ultrafast imaging
en_US
dc.title
Cortex‐wide microcirculation mapping with ultrafast large‐field multifocal illumination microscopy
en_US
dc.type
Journal Article
dc.date.published
2020-08-05
ethz.journal.title
Journal of Biophotonics
ethz.journal.volume
13
en_US
ethz.journal.issue
11
en_US
ethz.journal.abbreviated
J. biophotonics
ethz.pages.start
e202000198
en_US
ethz.size
7 p.
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Weinheim
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02140 - Dep. Inf.technologie und Elektrotechnik / Dep. of Inform.Technol. Electrical Eng.::02631 - Institut für Biomedizinische Technik / Institute for Biomedical Engineering::09648 - Razansky, Daniel / Razansky, Daniel
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02140 - Dep. Inf.technologie und Elektrotechnik / Dep. of Inform.Technol. Electrical Eng.::02631 - Institut für Biomedizinische Technik / Institute for Biomedical Engineering::09648 - Razansky, Daniel / Razansky, Daniel
ethz.date.deposited
2020-09-12T02:53:02Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2020-11-12T14:00:46Z
ethz.rosetta.lastUpdated
2020-11-12T14:00:46Z
ethz.rosetta.versionExported
true
ethz.COinS
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