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dc.contributor.author
Lima, Augusto M.
dc.contributor.author
Saint Auguste, Damian S.
dc.contributor.author
Cuenot, Francois
dc.contributor.author
Martins Cavaco, Ana C.
dc.contributor.author
Lachkar, Tom
dc.contributor.author
Khawand, Cindy M.E.
dc.contributor.author
Fraga-Silva, Rodrigo A.
dc.contributor.author
Stergiopulos, Nikolaos
dc.date.accessioned
2020-10-13T09:54:53Z
dc.date.available
2020-09-12T03:03:51Z
dc.date.available
2020-09-22T12:08:33Z
dc.date.available
2020-10-13T09:54:53Z
dc.date.issued
2020-09-07
dc.identifier.issn
1422-0067
dc.identifier.other
10.3390/ijms21186539
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/440150
dc.identifier.doi
10.3929/ethz-b-000440150
dc.description.abstract
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Platelets play a crucial role in the immunological response and are involved in the pathological settings of vascular diseases, and their adhesion to the extracellular matrix is important to bring leukocytes close to the endothelial cells and to form and stabilize the thrombus. Currently there are several methods to study platelet adhesion; however, the optimal parameters to perform the assay vary among studies, which hinders their comparison and reproducibility. Here, a standardization and validation of a fluorescence-based quantitative adhesion assay to study platelet-ECM interaction in a high-throughput screening format is proposed. Our study confirms that fluorescence-based quantitative assays can be effectively used to detect platelet adhesion, in which BCECF-AM presents the highest sensitivity in comparison to other dyes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Molecular Diversity Preservation International (MDPI)
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Standardization and validation of fluorescence-based quantitative assay to study human platelet adhesion to extracellular-matrix in a 384-well plate
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
International Journal of Molecular Sciences
ethz.journal.volume
21
en_US
ethz.journal.issue
18
en_US
ethz.journal.abbreviated
Int. j. mol. sci.
ethz.pages.start
6539
en_US
ethz.size
20 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Basel
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2020-09-12T03:04:03Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-09-22T12:08:53Z
ethz.rosetta.lastUpdated
2021-02-15T18:05:11Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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