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dc.contributor.author
Kooijman, Laurens
dc.contributor.author
Schuster, Matthias
dc.contributor.author
Baumann, Christian
dc.contributor.author
Jurt, Simon
dc.contributor.author
Löhr, Frank
dc.contributor.author
Fürtig, Boris
dc.contributor.author
Güntert, Peter
dc.contributor.author
Zerbe, Oliver
dc.date.accessioned
2020-11-13T08:21:34Z
dc.date.available
2020-09-20T04:16:17Z
dc.date.available
2020-09-21T12:14:08Z
dc.date.available
2020-11-13T08:21:34Z
dc.date.issued
2020-11-16
dc.identifier.issn
1433-7851
dc.identifier.issn
1521-3773
dc.identifier.issn
0570-0833
dc.identifier.other
10.1002/anie.202004393
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/441323
dc.description.abstract
To achieve efficient proton pumping in the light‐driven proton pump bacteriorhodopsin (bR), the protein must be tightly coupled to the retinal to rapidly convert retinal isomerization into protein structural rearrangements. Methyl group dynamics of bR embedded in lipid nanodiscs were determined in the dark‐adapted state, and were found to be mostly well ordered at the cytosolic side. Methyl groups in the M145A mutant of bR, which displays only 10 % residual proton pumping activity, are less well ordered, suggesting a link between side‐chain dynamics on the cytosolic side of the bR cavity and proton pumping activity. In addition, slow conformational exchange, attributed to low frequency motions of aromatic rings, was indirectly observed for residues on the extracellular side of the bR cavity. This may be related to reorganization of the water network. These observations provide a detailed picture of previously undescribed equilibrium dynamics on different time scales for ground‐state bR. © 2020 Wiley‐VCH GmbH.
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley
en_US
dc.subject
Dynamics
en_US
dc.subject
Membrane proteins
en_US
dc.subject
Retinal isomerization
en_US
dc.subject
Rhodopsin
en_US
dc.subject
Solution NMR
en_US
dc.title
Dynamics of Bacteriorhodopsin in the Dark‐Adapted State from Solution Nuclear Magnetic Resonance Spectroscopy
en_US
dc.type
Journal Article
dc.date.published
2020-07-29
ethz.journal.title
Angewandte Chemie. International Edition
ethz.journal.volume
59
en_US
ethz.journal.issue
47
en_US
ethz.journal.abbreviated
Angew. Chem. Int. Ed.
ethz.pages.start
20965
en_US
ethz.pages.end
20972
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Weinheim
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02515 - Laboratorium für Physikalische Chemie / Laboratory of Physical Chemistry::03782 - Riek, Roland / Riek, Roland
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02515 - Laboratorium für Physikalische Chemie / Laboratory of Physical Chemistry::03782 - Riek, Roland / Riek, Roland
ethz.date.deposited
2020-09-20T04:16:26Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2020-11-13T08:21:55Z
ethz.rosetta.lastUpdated
2021-02-15T20:44:25Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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