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dc.contributor.author
Behrendt, Lars
dc.contributor.author
Salek, M. Mehdi
dc.contributor.author
Trampe, Erik L.
dc.contributor.author
Fernandez, Vicente I.
dc.contributor.author
Lee, Kang Soo
dc.contributor.author
Kühl, Michael
dc.contributor.author
Stocker, Roman
dc.date.accessioned
2020-09-28T12:08:31Z
dc.date.available
2020-09-21T08:26:51Z
dc.date.available
2020-09-21T11:08:51Z
dc.date.available
2020-09-28T12:08:31Z
dc.date.issued
2020-09-02
dc.identifier.issn
2375-2548
dc.identifier.other
10.1126/sciadv.abb2754
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/441496
dc.identifier.doi
10.3929/ethz-b-000441496
dc.description.abstract
Photosynthetic microorganisms are key players in aquatic ecosystems with strong potential for bioenergy production, yet their systematic selection at the single-cell level for improved productivity or stress resilience (“phenotyping”) has remained largely inaccessible. To facilitate the phenotyping of microalgae and cyanobacteria, we developed “PhenoChip,” a platform for the multiparametric photophysiological characterization and selection of unicellular phenotypes under user-controlled physicochemical conditions. We used PhenoChip to expose single cells of the coral symbiont Symbiodinium to thermal and chemical treatments and monitor single-cell photophysiology via chlorophyll fluorometry. This revealed strain-specific thermal sensitivity thresholds and distinct pH optima for photosynthetic performance, and permitted the identification of single cells with elevated resilience toward rising temperature. Optical expulsion technology was used to collect single cells from PhenoChip, and their propagation revealed indications of transgenerational preservation of photosynthetic phenotypes. PhenoChip represents a versatile platform for the phenotyping of photosynthetic unicells relevant to biotechnology, ecotoxicology, and assisted evolution.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
American Association for the Advancement of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/4.0/
dc.title
PhenoChip: A single-cell phenomic platform for high-throughput photophysiological analyses of microalgae
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 4.0 International
ethz.journal.title
Science Advances
ethz.journal.volume
6
en_US
ethz.journal.issue
36
en_US
ethz.journal.abbreviated
Sci Adv
ethz.pages.start
eabb2754
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02115 - Dep. Bau, Umwelt und Geomatik / Dep. of Civil, Env. and Geomatic Eng.::02608 - Institut für Umweltingenieurwiss. / Institute of Environmental Engineering::09467 - Stocker, Roman / Stocker, Roman
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02205 - FIRST-Lab / FIRST Center for Micro- and Nanoscience
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02115 - Dep. Bau, Umwelt und Geomatik / Dep. of Civil, Env. and Geomatic Eng.::02608 - Institut für Umweltingenieurwiss. / Institute of Environmental Engineering::09467 - Stocker, Roman / Stocker, Roman
ethz.date.deposited
2020-09-21T08:26:58Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-09-21T11:09:08Z
ethz.rosetta.lastUpdated
2022-03-29T03:13:47Z
ethz.rosetta.versionExported
true
ethz.COinS
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