Show simple item record

dc.contributor.author
Sponchioni, Mattia
dc.contributor.author
Manfredini, Nicolo
dc.contributor.author
Zanoni, Arianna
dc.contributor.author
Scibona, Ernesto
dc.contributor.author
Morbidelli, Massimo
dc.contributor.author
Moscatelli, Davide
dc.date.accessioned
2020-10-13T15:32:11Z
dc.date.available
2020-10-11T05:07:46Z
dc.date.available
2020-10-13T15:32:11Z
dc.date.issued
2020-09-14
dc.identifier.issn
2373-9878
dc.identifier.other
10.1021/acsbiomaterials.0c01029
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/445418
dc.description.abstract
The efficacy of several cell therapy products is directly impacted by trypsinization, which can diminish the engrafting capacity of transplanted cells by cleaving cell surface receptors. Thermoresponsive surfaces can alleviate this drawback, enabling temperaturedriven and enzyme-free cell harvesting. However, the production of thermoresponsive surfaces relies on dedicated and complex equipment, often involving protocols dependent on high surface activation energies that prevent the development of scalable and universal platforms. In this work, we developed thermoresponsive copolymers incorporating styrene units that enable the copolymer adsorption on tissue culture polystyrene surfaces from an alcoholic solution in a short time, regardless of the vessel size and geometry, and without any particular equipment. In this way, the procedure can be performed with minimal effort by the end user on any surface. The thermoresponsive copolymers were synthesized via reversible addition-fragmentation chain transfer polymerization, providing high control over the polymer microstructure, a key parameter for tuning its cloud point and architecture. Block copolymers comprising a thermoresponsive segment and a polystyrene block exhibited optimal adhesion on conventional cell culture surfaces and permitted a more efficient temperature-mediated harvesting of adipose-derived stromal cells and Chinese hamster ovary cells compared to their statistical counterparts. To expand the application of this polymer deposition protocol to serum-free cell culture, we also considered the polymer modification with the tripeptide arginine-glycine-aspartic acid, known to promote the cell adhesion to synthetic substrates. The incorporation of this peptide enabled the collection in serum-free conditions of intact cell sheets from surfaces prepared shortly before their usage.
en_US
dc.language.iso
en
en_US
dc.publisher
American Chemical Society
en_US
dc.subject
thermoresponsive
en_US
dc.subject
cell sheet engineering
en_US
dc.subject
adsorption
en_US
dc.subject
smart surfaces
en_US
dc.subject
RGD
en_US
dc.subject
regenerative medicine
en_US
dc.title
Readily Adsorbable Thermoresponsive Polymers for the Preparation of Smart Cell-Culturing Surfaces on Site
en_US
dc.type
Journal Article
dc.date.published
2020-08-10
ethz.journal.title
ACS Biomaterials Science & Engineering
ethz.journal.volume
6
en_US
ethz.journal.issue
9
en_US
ethz.pages.start
5337
en_US
ethz.pages.end
5345
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2020-10-11T05:07:51Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2020-10-13T15:32:24Z
ethz.rosetta.lastUpdated
2020-10-13T15:32:24Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Readily%20Adsorbable%20Thermoresponsive%20Polymers%20for%20the%20Preparation%20of%20Smart%20Cell-Culturing%20Surfaces%20on%20Site&rft.jtitle=ACS%20Biomaterials%20Science%20&%20Engineering&rft.date=2020-09-14&rft.volume=6&rft.issue=9&rft.spage=5337&rft.epage=5345&rft.issn=2373-9878&rft.au=Sponchioni,%20Mattia&Manfredini,%20Nicolo&Zanoni,%20Arianna&Scibona,%20Ernesto&Morbidelli,%20Massimo&rft.genre=article&
 Search via SFX

Files in this item

FilesSizeFormatOpen in viewer

There are no files associated with this item.

Publication type

Show simple item record