Show simple item record

dc.contributor.author
Gerez, Juan A.
dc.contributor.author
Prymaczok, Natalia C.
dc.contributor.author
Riek, Roland
dc.contributor.editor
Kragelund, Birthe B.
dc.contributor.editor
Skriver, Karen
dc.date.accessioned
2020-10-16T06:51:24Z
dc.date.available
2020-10-13T10:52:46Z
dc.date.available
2020-10-16T06:46:00Z
dc.date.available
2020-10-16T06:51:24Z
dc.date.issued
2020
dc.identifier.isbn
978-1-0716-0523-3
en_US
dc.identifier.isbn
978-1-0716-0524-0
en_US
dc.identifier.issn
1064-3745
dc.identifier.issn
1940-6029
dc.identifier.other
10.1007/978-1-0716-0524-0_45
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/445714
dc.description.abstract
In-cell NMR enables structural insights at atomic resolution of proteins in their natural environment. To date, very few methods have been developed to study proteins by in-cell NMR in mammalian systems. Here we describe a detailed protocol to conduct in-cell NMR on the intrinsically disordered protein of alpha-Synuclein (αSyn) in mammalian cells. This chapter includes a simplified expression and purification protocol of recombinant αSyn and its delivery into mammalian cells. The chapter also describes how to assess the cell leakage that might occur to the cells, the setup of the instrument, and how to perform basic analyses with the obtained NMR data.
en_US
dc.language.iso
en
en_US
dc.publisher
Humana Press
en_US
dc.subject
IDP
en_US
dc.subject
Nuclear magnetic resonance
en_US
dc.subject
Electroporation
en_US
dc.subject
α-synuclein
en_US
dc.subject
Leakage
en_US
dc.title
In-Cell NMR of Intrinsically Disordered Proteins in Mammalian Cells
en_US
dc.type
Book Chapter
dc.date.published
2020-07-22
ethz.book.title
Intrinsically Disordered Proteins: Methods and Protocols
en_US
ethz.journal.title
Methods in Molecular Biology
ethz.journal.volume
2141
en_US
ethz.journal.abbreviated
Methods Mol Biol
ethz.pages.start
873
en_US
ethz.pages.end
893
en_US
ethz.identifier.scopus
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02515 - Laboratorium für Physikalische Chemie / Laboratory of Physical Chemistry::03782 - Riek, Roland / Riek, Roland
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02515 - Laboratorium für Physikalische Chemie / Laboratory of Physical Chemistry::03782 - Riek, Roland / Riek, Roland
en_US
ethz.date.deposited
2020-10-13T10:52:58Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2020-10-16T06:51:34Z
ethz.rosetta.lastUpdated
2020-10-16T06:51:34Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=In-Cell%20NMR%20of%20Intrinsically%20Disordered%20Proteins%20in%20Mammalian%20Cells&rft.jtitle=Methods%20in%20Molecular%20Biology&rft.date=2020&rft.volume=2141&rft.spage=873&rft.epage=893&rft.issn=1064-3745&1940-6029&rft.au=Gerez,%20Juan%20A.&Prymaczok,%20Natalia%20C.&Riek,%20Roland&rft.isbn=978-1-0716-0523-3&978-1-0716-0524-0&rft.genre=bookitem&rft_id=info:doi/978-1-0716-0523-3&info:doi/978-1-0716-0524-0&rft.btitle=Intrinsically%20Disordered%20Proteins:%20Methods%20and%20Protocols
 Search via SFX

Files in this item

FilesSizeFormatOpen in viewer

There are no files associated with this item.

Publication type

Show simple item record