Show simple item record

dc.contributor.author
Pereira, Fátima C.
dc.contributor.author
Wasmund, Kenneth
dc.contributor.author
Cobankovic, Iva
dc.contributor.author
Jehmlich, Nico
dc.contributor.author
Herbold, Craig W.
dc.contributor.author
Lee, Kang Soo
dc.contributor.author
Sziranyi, Barbara
dc.contributor.author
Vesely, Cornelia
dc.contributor.author
Decker, Thomas
dc.contributor.author
Stocker, Roman
dc.contributor.author
Warth, Benedikt
dc.contributor.author
von Bergen, Martin
dc.contributor.author
Wagner, Michael
dc.contributor.author
Berry, David
dc.date.accessioned
2020-10-19T10:01:33Z
dc.date.available
2020-10-16T03:42:00Z
dc.date.available
2020-10-19T10:01:33Z
dc.date.issued
2020
dc.identifier.issn
2041-1723
dc.identifier.other
10.1038/s41467-020-18928-1
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/446229
dc.identifier.doi
10.3929/ethz-b-000446229
dc.description.abstract
Many intestinal pathogens, including Clostridioides difficile, use mucus-derived sugars as crucial nutrients in the gut. Commensals that compete with pathogens for such nutrients are therefore ecological gatekeepers in healthy guts, and are attractive candidates for therapeutic interventions. Nevertheless, there is a poor understanding of which commensals use mucin-derived sugars in situ as well as their potential to impede pathogen colonization. Here, we identify mouse gut commensals that utilize mucus-derived monosaccharides within complex communities using single-cell stable isotope probing, Raman-activated cell sorting and mini-metagenomics. Sequencing of cell-sorted fractions reveals members of the underexplored family Muribaculaceae as major mucin monosaccharide foragers, followed by members of Lachnospiraceae, Rikenellaceae, and Bacteroidaceae families. Using this information, we assembled a five-member consortium of sialic acid and N-acetylglucosamine utilizers that impedes C. difficile’s access to these mucosal sugars and impairs pathogen colonization in antibiotic-treated mice. Our findings underscore the value of targeted approaches to identify organisms utilizing key nutrients and to rationally design effective probiotic mixtures.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Rational design of a microbial consortium of mucosal sugar utilizers reduces Clostridiodes difficile colonization
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2020-10-09
ethz.journal.title
Nature Communications
ethz.journal.volume
11
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Nat Commun
ethz.pages.start
5104
en_US
ethz.size
15 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02115 - Dep. Bau, Umwelt und Geomatik / Dep. of Civil, Env. and Geomatic Eng.::02608 - Institut für Umweltingenieurwiss. / Institute of Environmental Engineering::09467 - Stocker, Roman / Stocker, Roman
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02115 - Dep. Bau, Umwelt und Geomatik / Dep. of Civil, Env. and Geomatic Eng.::02608 - Institut für Umweltingenieurwiss. / Institute of Environmental Engineering::09467 - Stocker, Roman / Stocker, Roman
ethz.date.deposited
2020-10-16T03:42:05Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-10-19T10:01:46Z
ethz.rosetta.lastUpdated
2021-02-15T18:31:48Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Rational%20design%20of%20a%20microbial%20consortium%20of%20mucosal%20sugar%20utilizers%20reduces%20Clostridiodes%20difficile%20colonization&rft.jtitle=Nature%20Communications&rft.date=2020&rft.volume=11&rft.issue=1&rft.spage=5104&rft.issn=2041-1723&rft.au=Pereira,%20F%C3%A1tima%20C.&Wasmund,%20Kenneth&Cobankovic,%20Iva&Jehmlich,%20Nico&Herbold,%20Craig%20W.&rft.genre=article&rft_id=info:doi/10.1038/s41467-020-18928-1&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record