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dc.contributor.author
Heo, Millie
dc.contributor.author
Chenon, Guilhem
dc.contributor.author
Castrillon, Carlos
dc.contributor.author
Bibette, Jérôme
dc.contributor.author
Bruhns, Pierre
dc.contributor.author
Griffiths, Andrew D.
dc.contributor.author
Baudry, Jean
dc.contributor.author
Eyer, Klaus
dc.date.accessioned
2020-11-03T15:43:53Z
dc.date.available
2020-11-03T03:58:12Z
dc.date.available
2020-11-03T15:43:53Z
dc.date.issued
2020-10-26
dc.identifier.issn
2399-3642
dc.identifier.other
10.1038/s42003-020-01296-3
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/449371
dc.identifier.doi
10.3929/ethz-b-000449371
dc.description.abstract
Antibodies with antibacterial activity need to bind to the bacterial surface with affinity, specificity, and sufficient density to induce efficient elimination. To characterize the anti-bacterial antibody repertoire, we developed an in-droplet bioassay with single-antibody resolution. The assay not only allowed us to identify whether the secreted antibodies recognized a bacterial surface antigen, but also to estimate the apparent dissociation constant (KD app) of the interaction and the density of the recognized epitope on the bacteria. Herein, we found substantial differences within the KD app/epitope density profiles in mice immunized with various species of heat-killed bacteria. The experiments further revealed a high cross-reactivity of the secreted IgG repertoires, binding to even unrelated bacteria with high affinity. This application confirmed the ability to quantify the anti-bacterial antibody repertoire and the utility of the developed bioassay to study the interplay between bacteria and the humoral response. © 2020, The Author(s).
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Springer
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Deep phenotypic characterization of immunization-induced antibacterial IgG repertoires in mice using a single-antibody bioassay
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Communications Biology
ethz.journal.volume
3
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Commun Biol
ethz.pages.start
614
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
High-throughput single-cell phenotypic analysis of functional antibody repertoires.
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::09676 - Eyer, Klaus (ehemalig) / Eyer, Klaus (former)
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::09676 - Eyer, Klaus (ehemalig) / Eyer, Klaus (former)
ethz.grant.agreementno
803363
ethz.grant.fundername
EC
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.program
H2020
ethz.date.deposited
2020-11-03T03:58:22Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-11-03T15:44:03Z
ethz.rosetta.lastUpdated
2024-02-02T12:25:46Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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