Expression, Purification, and Functional Characterization of Tectonin 2 from Laccaria bicolor: A Six-Bladed Beta-Propeller Lectin Specific for O-Methylated Glycans
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Date
2020Type
- Book Chapter
Abstract
Tectonins are conserved defense proteins of innate immune systems featuring a β-propeller fold. Tectonin 2 from Laccaria bicolor, Lb-Tec2, is the first fungal representative of the tectonin superfamily that has been described. In-depth characterization revealed a specificity for O-methylated glycans and identified a unique sequence motif and binding site architecture underlying this unusual specificity. This chapter provides information on how to produce and purify recombinant Lb-Tec2, characterize its interaction with O-methylated glycans and demonstrate its biological function. Show more
Publication status
publishedBook title
Lectin Purification and Analysis: Methods and ProtocolsJournal / series
Methods in Molecular BiologyVolume
Pages / Article No.
Publisher
Humana PressSubject
β-Propeller; Non-self recognition; O-Methylated glycans; Defense effector; Nematotoxic lectin; Innate immunityOrganisational unit
08838 - Künzler, Markus (Tit.-Prof.)
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