Expression, Purification, and Functional Characterization of Tectonin 2 from Laccaria bicolor: A Six-Bladed Beta-Propeller Lectin Specific for O-Methylated Glycans
dc.contributor.author
Wohlschlager, Therese
dc.contributor.author
Titz, Alexander
dc.contributor.author
Künzler, Markus
dc.contributor.author
Varrot, Annabelle
dc.date.accessioned
2020-11-20T08:04:02Z
dc.date.available
2020-11-03T13:18:06Z
dc.date.available
2020-11-04T07:14:58Z
dc.date.available
2020-11-04T07:18:31Z
dc.date.available
2020-11-20T08:04:02Z
dc.date.issued
2020
dc.identifier.isbn
978-1-0716-0429-8
en_US
dc.identifier.isbn
978-1-0716-0430-4
en_US
dc.identifier.issn
1064-3745
dc.identifier.issn
1940-6029
dc.identifier.other
10.1007/978-1-0716-0430-4_58
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/449501
dc.description.abstract
Tectonins are conserved defense proteins of innate immune systems featuring a β-propeller fold. Tectonin 2 from Laccaria bicolor, Lb-Tec2, is the first fungal representative of the tectonin superfamily that has been described. In-depth characterization revealed a specificity for O-methylated glycans and identified a unique sequence motif and binding site architecture underlying this unusual specificity. This chapter provides information on how to produce and purify recombinant Lb-Tec2, characterize its interaction with O-methylated glycans and demonstrate its biological function.
en_US
dc.language.iso
en
en_US
dc.publisher
Humana Press
en_US
dc.subject
β-Propeller
en_US
dc.subject
Non-self recognition
en_US
dc.subject
O-Methylated glycans
en_US
dc.subject
Defense effector
en_US
dc.subject
Nematotoxic lectin
en_US
dc.subject
Innate immunity
en_US
dc.title
Expression, Purification, and Functional Characterization of Tectonin 2 from Laccaria bicolor: A Six-Bladed Beta-Propeller Lectin Specific for O-Methylated Glycans
en_US
dc.type
Book Chapter
dc.date.published
2020-04-19
ethz.book.title
Lectin Purification and Analysis: Methods and Protocols
en_US
ethz.journal.title
Methods in Molecular Biology
ethz.journal.volume
2132
en_US
ethz.journal.abbreviated
Methods Mol Biol
ethz.pages.start
669
en_US
ethz.pages.end
682
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::08838 - Künzler, Markus (Tit.-Prof.)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::08838 - Künzler, Markus (Tit.-Prof.)
en_US
ethz.date.deposited
2020-11-03T13:18:14Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2020-11-04T07:18:41Z
ethz.rosetta.lastUpdated
2022-03-29T04:03:44Z
ethz.rosetta.versionExported
true
ethz.COinS
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