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dc.contributor.author
Schlotter, Tilman
dc.contributor.author
Weaver, Sean
dc.contributor.author
Forró, Csaba
dc.contributor.author
Momotenko, Dmitry
dc.contributor.author
Vörös, Janos
dc.contributor.author
Zambelli, Tomaso
dc.contributor.author
Aramesh, Morteza
dc.date.accessioned
2021-09-13T12:07:16Z
dc.date.available
2020-11-09T04:12:09Z
dc.date.available
2020-11-09T11:47:16Z
dc.date.available
2021-03-12T11:29:24Z
dc.date.available
2021-03-12T13:00:53Z
dc.date.available
2021-09-13T12:07:16Z
dc.date.issued
2020-10-27
dc.identifier.issn
1936-0851
dc.identifier.issn
1936-086X
dc.identifier.other
10.1021/acsnano.0c04281
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/450209
dc.identifier.doi
10.3929/ethz-b-000450209
dc.description.abstract
Nanopore sensing of single nucleotides has emerged as a promising single-molecule technology for DNA sequencing and proteomics. Despite the conceptual simplicity of nanopores, adoption of this technology for practical applications has been limited by a lack of pore size adjustability and an inability to perform long-term recordings in complex solutions. Here we introduce a method for fast and precise on-demand formation of a nanopore with controllable size between 2 and 20 nm through force-controlled adjustment of the nanospace formed between the opening of a microfluidic device (made of silicon nitride) and a soft polymeric substrate. The introduced nanopore system enables stable measurements at arbitrary locations. By accurately positioning the nanopore in the proximity of single neurons and continuously recording single-molecule translations over several hours, we have demonstrated this is a powerful approach for single-cell proteomics and secretomics.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
American Chemical Society
en_US
dc.rights.uri
http://rightsstatements.org/page/InC-NC/1.0/
dc.subject
dynamic nanopores
en_US
dc.subject
single-cell proteomics
en_US
dc.subject
biomolecular sensors
en_US
dc.subject
AFM-integrated nanopores
en_US
dc.subject
nanoconfinement of biomolecules
en_US
dc.title
Force-Controlled Formation of Dynamic Nanopores for Single-Biomolecule Sensing and Single-Cell Secretomics
en_US
dc.type
Journal Article
dc.rights.license
In Copyright - Non-Commercial Use Permitted
dc.date.published
2020-09-11
ethz.journal.title
ACS Nano
ethz.journal.volume
14
en_US
ethz.journal.issue
10
en_US
ethz.journal.abbreviated
ACS Nano
ethz.pages.start
12993
en_US
ethz.pages.end
13003
en_US
ethz.size
32 p. accepted version
en_US
ethz.version.deposit
acceptedVersion
en_US
ethz.grant
Single Entities at High Magnification: Mapping, Measuring and Manipulating Nanoparticles
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Columbus, OH
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
ethz.grant.agreementno
174217
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Ambizione
ethz.date.deposited
2020-11-09T04:12:30Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.date.embargoend
2021-09-11
ethz.rosetta.installDate
2020-11-09T11:47:52Z
ethz.rosetta.lastUpdated
2021-02-15T20:38:08Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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