Show simple item record

dc.contributor.author
Capossela, Simona
dc.contributor.author
Mathew, Vikas
dc.contributor.author
Boos, Manuela
dc.contributor.author
Bertolo, Alessandro
dc.contributor.author
Krupkova, Olga
dc.contributor.author
Stoyanov, Jivko V.
dc.date.accessioned
2020-11-13T06:56:17Z
dc.date.available
2020-11-11T06:14:25Z
dc.date.available
2020-11-13T06:56:17Z
dc.date.issued
2020-10-28
dc.identifier.other
10.2147/DDDT.S258368
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/450692
dc.identifier.doi
10.3929/ethz-b-000450692
dc.description.abstract
Purpose: The production of nano-erythrosomes (NEs) by extrusion, which is considered the "gold standard", has several disadvantages such as difficult equipment assembly, long procedure time, variable pressure, and problems with sterility. An alternative approach, using ultrasound probe, has been shown to overheat the sample and have suboptimal results compared to the extrusion method. In our study, we propose, develop, and test a new method for the fabrication of NEs based on shear force and then compare it to the "gold standard" extrusion approach. Methods: The new method consists of mechanical shear force disruption of the hemoglobin-depleted erythrocyte ghost membranes, with the aid of a rotor stator based tissue homogenizer. Using the same batches of erythrocyte ghost membranes, we compared NEs produced by shear force to NEs produced by the well-established extrusion approach. NEs were characterized for yield, size, encapsulation efficiency, morphology, and stability by flow cytometry (FC), transmission electron microscopy (TEM), and zeta potential analysis. Results: The shear force based process was easier to set up, significantly faster, had better sterility control, and decreased variability between batches. The shear force method generated NEs with the desired size distribution (particles diameter similar to 125 nm), which were morphologically and functionally equivalent to the NEs produced by extrusion. NEs produced by shear force were stable in terms of counts, size, and fluorescence intensity for 3 weeks at +4 degrees C. Moreover, they showed colloidal stability and minimal influence to centrifugal stress, turbulence shock, and hemolytic potential. Conclusion: The newly proposed shear force method allows faster, easier, and highly reproducible NEs production when compared to the conventional extrusion approach. The new setup allows simultaneous production of sterile batches of NEs, which have homogenous size distribution, good stability, and improved shelf life storage. The ability of the shear force method to process also high concentration samples indicates a future potential development of large-scale NEs production and industrial application, which has been a challenge for the extrusion method.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Dove Medical Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/3.0/
dc.subject
erythrocytes
en_US
dc.subject
nanotechnology
en_US
dc.subject
nanoerythrosomes
en_US
dc.subject
extrusion
en_US
dc.subject
shear force
en_US
dc.subject
new method
en_US
dc.title
Novel Fast and Reliable Method for Nano-Erythrosome Production Using Shear Force
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 3.0 Unported
dc.date.published
2020-10-07
ethz.journal.title
DRUG DESIGN DEVELOPMENT AND THERAPY
ethz.journal.volume
14
en_US
ethz.pages.start
4547
en_US
ethz.pages.end
4560
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.publication.place
Albany
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2020-11-11T06:14:29Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-11-13T06:56:36Z
ethz.rosetta.lastUpdated
2021-02-15T20:43:52Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Novel%20Fast%20and%20Reliable%20Method%20for%20Nano-Erythrosome%20Production%20Using%20Shear%20Force&rft.jtitle=DRUG%20DESIGN%20DEVELOPMENT%20AND%20THERAPY&rft.date=2020-10-28&rft.volume=14&rft.spage=4547&rft.epage=4560&rft.au=Capossela,%20Simona&Mathew,%20Vikas&Boos,%20Manuela&Bertolo,%20Alessandro&Krupkova,%20Olga&rft.genre=article&rft_id=info:doi/10.2147/DDDT.S258368&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record