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dc.contributor.author
Azadi, Afsoon S.
dc.contributor.author
Carmichael, Ruth E.
dc.contributor.author
Kovacs, Werner J.
dc.contributor.author
Koster, Janet
dc.contributor.author
Kors, Suzan
dc.contributor.author
Waterham, Hans R.
dc.contributor.author
Schrader, Michael
dc.date.accessioned
2020-11-19T10:48:47Z
dc.date.available
2020-11-13T18:52:59Z
dc.date.available
2020-11-19T10:48:47Z
dc.date.issued
2020-10
dc.identifier.issn
2296-634X
dc.identifier.other
10.3389/fcell.2020.577637
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/451344
dc.identifier.doi
10.3929/ethz-b-000451344
dc.description.abstract
In mammals, peroxisomes perform crucial functions in cellular metabolism, signaling and viral defense which are essential to the viability of the organism. Molecular cues triggered by changes in the cellular environment induce a dynamic response in peroxisomes, which manifests itself as a change in peroxisome number, altered enzyme levels and adaptations to the peroxisomal morphology. How the regulation of this process is integrated into the cell’s response to different stimuli, including the signaling pathways and factors involved, remains unclear. Here, a cell-based peroxisome proliferation assay has been applied to investigate the ability of different stimuli to induce peroxisome proliferation. We determined that serum stimulation, long-chain fatty acid supplementation and TGFβ application all increase peroxisome elongation, a prerequisite for proliferation. Time-resolved mRNA expression during the peroxisome proliferation cycle revealed a number of peroxins whose expression correlated with peroxisome elongation, including the β isoform of PEX11, but not the α or γ isoforms. An initial map of putative regulatory motif sites in the respective promoters showed a difference between binding sites in PEX11α and PEX11β, suggesting that these genes may be regulated by distinct pathways. A functional SMAD2/3 binding site in PEX11β points to the involvement of the TGFβ signaling pathway in expression of this gene and thus peroxisome proliferation/dynamics in humans.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Media
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
peroxisomes
en_US
dc.subject
organelle dynamics
en_US
dc.subject
transcriptional regulation
en_US
dc.subject
peroxin
en_US
dc.subject
PEX11
en_US
dc.subject
transforming growth factor beta
en_US
dc.title
A Functional SMAD2/3 Binding Site in the PEX11β Promoter Identifies a Role for TGFβ in Peroxisome Proliferation in Humans
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2020-10-23
ethz.journal.title
Frontiers in Cell and Developmental Biology
ethz.journal.volume
8
en_US
ethz.journal.abbreviated
Front. Cell Dev. Biol.
ethz.pages.start
577637
en_US
ethz.size
18 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Exploring the molecular mechanism of Hif-2alpha-mediated pexophagy and the role of pexophagy in tumorigenesis
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Lausanne
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::03630 - Krek, Wilhelm (ehemalig) / Krek, Wilhelm (former)
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::03630 - Krek, Wilhelm (ehemalig) / Krek, Wilhelm (former)
ethz.grant.agreementno
166245
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projekte Lebenswissenschaften
ethz.date.deposited
2020-11-13T18:53:11Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-11-19T10:48:58Z
ethz.rosetta.lastUpdated
2024-02-02T12:31:06Z
ethz.rosetta.versionExported
true
ethz.COinS
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