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dc.contributor.author
Zilliox, Michael J.
dc.contributor.author
Gange, William S.
dc.contributor.author
Kuffel, Gina
dc.contributor.author
Roese Mores, Carine
dc.contributor.author
Joyce, Cara
dc.contributor.author
de Bustros, Paul
dc.contributor.author
Bouchard, Charles S.
dc.date.accessioned
2020-11-19T09:43:26Z
dc.date.available
2020-11-18T15:15:01Z
dc.date.available
2020-11-19T09:43:26Z
dc.date.issued
2020-10
dc.identifier.issn
1542-0124
dc.identifier.other
10.1016/j.jtos.2020.07.007
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/451914
dc.description.abstract
Purpose There is growing evidence for a critical role of the microbiome in ocular health and disease. We performed a prospective, observational study to characterize the ocular surface microbiome (OSM) in four chronic ocular surface diseases (OSDs) and healthy controls. Methods Sterile swabs were used to collect samples from each eye of 39 patients (78 eyes). Sterile technique and multiple controls were used to assess contamination during DNA extraction, amplification and sequencing. Concurrent use of topical antibiotics, steroids, and bandage contact lenses (BCLs) was documented. Results Despite the low biomass of the ocular surface, 47/78 (60%) eyes sampled had positive sequencing reads. We observed that half of patients (8/17, 47%) had distinct microbiomes in each eye. Healthy controls had a Lactobacillus/Streptococcus mixture or significant Corynebacterium. Staphylococcus predominated in 4/7 (57%) patients with Stevens-Johnson Syndrome (SJS) in at least one eye, compared to 0/10 healthy controls. Interestingly, 8/11 (73%) eyes with SJS were using BCLs, including 4/5 (80%) eyes dominated by Staphylococcus. Lax eyelid syndrome (LES) and Dry Eye Disease (DED) patients had similar OSMs, with Corynebacterium being the most prevalent bacteria. Alpha diversity was higher in controls and ocular graft-vs-host (oGVHD) patients compared to the other OSDs. Conclusions Only 50% of the 39 patients had similar microbiomes in each eye. A majority of healthy eyes had a Lactobacillus/Streptococcus mix or Corynebacterium microbiome. Staphylococcus predominated in SJS, Lactobacillus in oGVHD, and Corynebacterium in DED and LES. There may be an association between different OSDs and the microbiome.
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.subject
Microbiome
en_US
dc.subject
SJS
en_US
dc.subject
oGVHD
en_US
dc.subject
Dry eye
en_US
dc.subject
Floppy eyelid syndrome
en_US
dc.title
Assessing the ocular surface microbiome in severe ocular surface diseases
en_US
dc.type
Journal Article
dc.date.published
2020-07-24
ethz.journal.title
The Ocular Surface
ethz.journal.volume
18
en_US
ethz.journal.issue
4
en_US
ethz.pages.start
706
en_US
ethz.pages.end
712
en_US
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology
en_US
ethz.date.deposited
2020-11-18T15:15:11Z
ethz.source
FORM
ethz.eth
no
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2020-11-19T09:43:39Z
ethz.rosetta.lastUpdated
2021-02-15T20:50:22Z
ethz.rosetta.versionExported
true
ethz.COinS
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