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dc.contributor.author
Rattray, Jayne E.
dc.contributor.author
Smittenberg, Rienk
dc.date.accessioned
2020-12-10T12:59:01Z
dc.date.available
2020-12-09T15:59:22Z
dc.date.available
2020-12-10T12:59:01Z
dc.date.issued
2020-09-18
dc.identifier.issn
2296-7745
dc.identifier.other
10.3389/fmars.2020.539601
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/455576
dc.identifier.doi
10.3929/ethz-b-000455576
dc.description.abstract
Over the last decade, glycerol dialkyl glycerol tetraethers (GDGTs) have become one of the most investigated lipid classes in marine and terrestrial organic geochemical research. GDGTs are microbial membrane core lipids biosynthesized as multiple homolog series of isoprenoid or methyl-branched isomers [isoprenoid glycerol dialkyl glycerol tetraethers (isoGDGTs) and Branched GDGTs (brGDGTs), respectively], whose relative abundance depend on a range of environmental parameters, including temperature. This has led to the development of GDGT-based temperature proxies. A key aspect in the analysis of GDGTs and the further development of their use as environmental proxies is good chromatographic separation of the full range of structural and stereo-isomers, with potential for discovery of novel GDGT variants. Several HPLC methods have been developed to this extent, but partial co-elution of GDGTs remains an issue despite long run times. In this study, we investigate the effects of different types of reverse phase (RP) chromatography on the separation of GDGT isomers. We found that the use of a Kinetex C18-XB column gives good separation of isoGDGT isomers in comparison to the recently developed double column HILIC analysis operated in normal phase (NP) and has a shorter run time. In marine samples, the regularly reported isoprenoid GDGTs separated in a similar way as in NP, however an earlier eluting group was observed to elute with the crenarchaeol isomer used in the TEX86 proxy. In a Swedish peat bog sample, a large range of isoGDGT isomers were observed. We observed a range of brGDGT isomers in several samples often with near baseline separation. Exact identification of all these isomers remained elusive, due to the different mechanism of separation in RP, and the complexity of the brGDGT family. The C18-XB method is rapid and versatile and can be set up on either low-pressure HPLC systems (max 400 bar) with a sample run time of 25 min for brGDGTs and 45 min to include isoGDGTs. On UHPLC-MS systems (>600 bar) the sample run time is reduced to 15 min. Most importantly, the C18-XB method presented here gives unusual separation of both isoprenoid and brGDGTs and could be a useful tool for the further elucidation of the biological sources and environmental factors that play a role in the production of different GDGT isomers.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Media
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
tetraethers
en_US
dc.subject
HPLC-MS
en_US
dc.subject
isomer resolution
en_US
dc.subject
reverse phase
en_US
dc.subject
GDGT
en_US
dc.title
Separation of Branched and Isoprenoid Glycerol Dialkyl Glycerol Tetraether (GDGT) Isomers in Peat Soils and Marine Sediments Using Reverse Phase Chromatography
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Frontiers in Marine Science
ethz.journal.volume
7
en_US
ethz.pages.start
539601
en_US
ethz.size
17 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.publication.place
Lausanne
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02330 - Dep. Erdwissenschaften / Dep. of Earth Sciences::02704 - Geologisches Institut / Geological Institute::03868 - Eglinton, Timothy I. / Eglinton, Timothy I.
en_US
ethz.date.deposited
2020-12-09T15:59:34Z
ethz.source
FORM
ethz.eth
no
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-12-10T12:59:12Z
ethz.rosetta.lastUpdated
2021-02-15T21:44:00Z
ethz.rosetta.versionExported
true
ethz.COinS
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