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dc.contributor.author
Macdonald, Duncan S.
dc.contributor.author
Hilvert, Donald
dc.contributor.editor
Tawfik, Dan S.
dc.date.accessioned
2021-01-26T06:39:07Z
dc.date.available
2021-01-01T14:08:36Z
dc.date.available
2021-01-26T06:37:39Z
dc.date.available
2021-01-26T06:39:07Z
dc.date.issued
2020
dc.identifier.isbn
978-0-12-824431-9
en_US
dc.identifier.issn
0076-6879
dc.identifier.other
10.1016/bs.mie.2020.06.014
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/458803
dc.description.abstract
Directed evolution has emerged as a powerful technique for the rapid tailoring of enzymes toward particular synthetic demands, spawning a number of enzymes capable of complex chemical transformations. During random mutagenesis of a protein, changes in fitness must be assayed in order to quantify and understand the relative effect a given mutation has, and the assay employed must be carefully chosen to report directly on the transformation of interest. Here, we describe a series of medium-throughput screening techniques that have been utilized for the evolution and engineering of an artificial carboligase, RA95.5-8, resulting in improvement of catalytic efficiency of a number of promiscuous chemistries. The methods make use of common analytical chemistry equipment and low-cost materials, and may help inspire development of novel screening workflows for related transformations.
en_US
dc.language.iso
en
en_US
dc.publisher
Academic Press
en_US
dc.subject
Directed evolution
en_US
dc.subject
Protein engineering
en_US
dc.subject
Screening
en_US
dc.subject
Assay
en_US
dc.subject
Artificial enzymes
en_US
dc.subject
Carboligase
en_US
dc.subject
HPLC
en_US
dc.subject
UV/Vis
en_US
dc.title
Enhancing promiscuous chemistries of a Schiff-base forming enzyme by divergent evolution
en_US
dc.type
Book Chapter
dc.date.published
2020-07-11
ethz.book.title
Enzyme Engineering and Evolution: Specific Enzyme Applications
en_US
ethz.journal.title
Methods in Enzymology
ethz.journal.volume
644
en_US
ethz.pages.start
95
en_US
ethz.pages.end
120
en_US
ethz.identifier.wos
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02514 - Laboratorium für Organische Chemie / Laboratory of Organic Chemistry::03492 - Hilvert, Donald (emeritus) / Hilvert, Donald (emeritus)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02514 - Laboratorium für Organische Chemie / Laboratory of Organic Chemistry::03492 - Hilvert, Donald (emeritus) / Hilvert, Donald (emeritus)
en_US
ethz.date.deposited
2021-01-01T14:08:44Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2021-01-26T06:37:49Z
ethz.rosetta.lastUpdated
2022-03-29T04:58:15Z
ethz.rosetta.versionExported
true
ethz.COinS
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