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dc.contributor.author
Karow, Marisa
dc.contributor.author
Camp, J. Gray
dc.contributor.author
Falk, Sven
dc.contributor.author
Gerber, Tobias
dc.contributor.author
Pataskar, Abhijeet
dc.contributor.author
Gac-Santel, Malgorzata
dc.contributor.author
Kageyama, Jorge
dc.contributor.author
Brazovskaja, Agnieska
dc.contributor.author
Garding, Angela
dc.contributor.author
Fan, Wenqiang
dc.contributor.author
Riedemann, Therese
dc.contributor.author
Casamassa, Antonella
dc.contributor.author
Smiyakin, Andrej
dc.contributor.author
Schichor, Christian
dc.contributor.author
Götz, Magdalena
dc.contributor.author
Tiwari, Vijay K.
dc.contributor.author
Treutlein, Barbara
dc.contributor.author
Berninger, Benedikt
dc.date.accessioned
2021-01-25T15:46:48Z
dc.date.available
2021-01-06T07:48:16Z
dc.date.available
2021-01-25T15:46:48Z
dc.date.issued
2018-07
dc.identifier.issn
1097-6256
dc.identifier.issn
1546-1726
dc.identifier.other
10.1038/s41593-018-0168-3
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/459748
dc.description.abstract
Ectopic expression of defined transcription factors can force direct cell-fate conversion from one lineage to another in the absence of cell division. Several transcription factor cocktails have enabled successful reprogramming of various somatic cell types into induced neurons (iNs) of distinct neurotransmitter phenotype. However, the nature of the intermediate states that drive the reprogramming trajectory toward distinct iN types is largely unknown. Here we show that successful direct reprogramming of adult human brain pericytes into functional iNs by Ascl1 and Sox2 encompasses transient activation of a neural stem cell-like gene expression program that precedes bifurcation into distinct neuronal lineages. During this transient state, key signaling components relevant for neural induction and neural stem cell maintenance are regulated by and functionally contribute to iN reprogramming and maturation. Thus, Ascl1- and Sox2-mediated reprogramming into a broad spectrum of iN types involves the unfolding of a developmental program via neural stem cell-like intermediates.
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.title
Direct pericyte-to-neuron reprogramming via unfolding of a neural stem cell-like program
en_US
dc.type
Journal Article
dc.date.published
2018-06-18
ethz.journal.title
Nature Neuroscience
ethz.journal.volume
21
en_US
ethz.journal.issue
7
en_US
ethz.journal.abbreviated
Nat. neurosci.
ethz.pages.start
932
en_US
ethz.pages.end
940
en_US
ethz.grant
Reconstructing human cortex development and malformation with single-cell transcriptomics
en_US
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::09485 - Treutlein, Barbara / Treutlein, Barbara
en_US
ethz.grant.agreementno
758877
ethz.grant.fundername
EC
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.program
H2020
ethz.date.deposited
2021-01-06T07:48:23Z
ethz.source
FORM
ethz.eth
no
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2021-01-25T15:46:57Z
ethz.rosetta.lastUpdated
2021-01-25T15:46:57Z
ethz.rosetta.versionExported
true
ethz.COinS
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