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dc.contributor.author
Jiang, Xi
dc.contributor.author
Payá-Tormo, Lucía
dc.contributor.author
Coroian, Diana
dc.contributor.author
García-Rubio, Inés
dc.contributor.author
Castellanos-Rueda, Rocío
dc.contributor.author
Eseverri, Álvaro
dc.contributor.author
López-Torrejón, Gema
dc.contributor.author
Burén, Stefan
dc.contributor.author
Rubio, Luis M.
dc.date.accessioned
2021-01-12T12:13:51Z
dc.date.available
2021-01-09T15:00:54Z
dc.date.available
2021-01-12T12:13:14Z
dc.date.available
2021-01-12T12:13:51Z
dc.date.issued
2021
dc.identifier.issn
2399-3642
dc.identifier.other
10.1038/s42003-020-01536-6
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/460871
dc.identifier.doi
10.3929/ethz-b-000460871
dc.description.abstract
Engineering nitrogen fixation in eukaryotes requires high expression of functional nitrogenase structural proteins, a goal that has not yet been achieved. Here we build a knowledge-based library containing 32 nitrogenase nifH sequences from prokaryotes of diverse ecological niches and metabolic features and combine with rapid screening in tobacco to identify superior NifH variants for plant mitochondria expression. Three NifH variants outperform in tobacco mitochondria and are further tested in yeast. Hydrogenobacter thermophilus (Aquificae) NifH is isolated in large quantities from yeast mitochondria and fulfills NifH protein requirements for efficient N2 fixation, including electron transfer for substrate reduction, P-cluster maturation, and FeMo-co biosynthesis. H. thermophilus NifH expressed in tobacco leaves shows lower nitrogenase activity than that from yeast. However, transfer of [Fe4S4] clusters from NifU to NifH in vitro increases 10-fold the activity of the tobacco-isolated NifH, revealing that plant mitochondria [Fe-S] cluster availability constitutes a bottleneck to engineer plant nitrogenases.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Exploiting genetic diversity and gene synthesis to identify superior nitrogenase NifH protein variants to engineer N2-fixation in plants
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-01-04
ethz.journal.title
Communications Biology
ethz.journal.volume
4
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Commun Biol
ethz.pages.start
4
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
ethz.publication.status
published
en_US
ethz.date.deposited
2021-01-09T15:01:14Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-01-12T12:13:22Z
ethz.rosetta.lastUpdated
2024-02-02T12:50:15Z
ethz.rosetta.versionExported
true
ethz.COinS
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