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dc.contributor.author
Chibani, Cynthia M.
dc.contributor.author
Roth, Olivia
dc.contributor.author
Liesegang, Heiko
dc.contributor.author
Wendling, Carolin Charlotte
dc.date.accessioned
2022-02-10T16:29:44Z
dc.date.available
2021-01-14T13:28:29Z
dc.date.available
2021-02-25T20:32:41Z
dc.date.available
2022-02-10T16:29:44Z
dc.date.issued
2020-04-15
dc.identifier.other
10.21203/rs.2.23653/v2
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/462582
dc.description.abstract
Background Species of the genus Vibrio, one of the most diverse bacteria genera, have undergone niche adaptation followed by clonal expansion. Niche adaptation and ultimately the formation of ecotypes and speciation in this genus has been suggested to be mainly driven by horizontal gene transfer (HGT) through mobile genetic elements (MGEs). Our knowledge about the diversity and distribution of Vibrio MGEs is heavily biased towards human pathogens and our understanding of the distribution of core genomic signatures and accessory genes encoded on MGEs within specific Vibrio clades is still incomplete. We used nine different strains of the marine bacterium Vibrio alginolyticus isolated from pipefish in the Kiel-Fjord to perform a multiscale-comparative genomic approach that allowed us to investigate (1) those genomic signatures that characterize a habitat-specific ecotype and (2) the source of genomic variation within this ecotype. Results We found that the nine isolates from the Kiel-Fjord have a closed-pangenome and did not differ based on core-genomic signatures. Unique genomic regions and a unique repertoire of MGEs within the Kiel-Fjord isolates suggest that the acquisition of gene-blocks by HGT played an important role in the evolution of this ecotype. Additionally, we found that ~90% of the genomic variation among the nine isolates is encoded on MGEs, which supports ongoing theory that accessory genes are predominately located on MGEs and shared by HGT. Lastly, we could show that these nine isolates share a unique virulence and resistance profile which clearly separates them from all other investigated V. alginolyticus strains and suggests that these are habitat-specific genes, required for a successful colonization of the pipefish, the niche of this ecotype. Conclusion We conclude that all nine V. alginolyticus strains from the Kiel-Fjord belong to a unique ecotype, which we named the Kiel-alginolyticus ecotype. The low sequence variation of the core-genome in combination with the presence of MGE encoded relevant traits, as well as the presence of a suitable niche (here the pipefish), suggest, that this ecotype might have evolved from a clonal expansion following HGT driven niche-adaptation.
en_US
dc.language.iso
en
en_US
dc.publisher
Research Square
en_US
dc.subject
Pangenome
en_US
dc.subject
Mega-plasmids
en_US
dc.subject
Mobile genetic elements
en_US
dc.subject
Horizontal gene transfer
en_US
dc.subject
Genomic islands
en_US
dc.subject
Niche-adaptation
en_US
dc.title
Genomic variation among closely related Vibrio alginolyticus strains is located on mobile genetic elements
en_US
dc.type
Working Paper
ethz.journal.title
Research Square
ethz.size
21 p.
en_US
ethz.publication.place
Durham, NC
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::09497 - Hall, Alex / Hall, Alex
en_US
ethz.identifier.orcidWorkCode
72138556
ethz.relation.isPreviousVersionOf
10.3929/ethz-b-000416199
ethz.date.deposited
2021-01-14T13:28:38Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.version
Version 2
en_US
ethz.rosetta.installDate
2022-02-10T16:30:26Z
ethz.rosetta.lastUpdated
2022-03-29T18:45:34Z
ethz.rosetta.versionExported
true
ethz.COinS
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