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dc.contributor.author
Holloway, Emily M.
dc.contributor.author
Czerwinski, Michael
dc.contributor.author
Tsai, Yu-Hwai
dc.contributor.author
Wu, Joshua H.
dc.contributor.author
Wu, Angeline
dc.contributor.author
Childs, Charlie J.
dc.contributor.author
Walton, Katherine D.
dc.contributor.author
Sweet, Caden W.
dc.contributor.author
Yu, Qianhui
dc.contributor.author
Glass, Ian
dc.contributor.author
Treutlein, Barbara
dc.contributor.author
Camp, J. Gray
dc.contributor.author
Spence, Jason R.
dc.date.accessioned
2021-03-05T14:39:27Z
dc.date.available
2021-01-21T08:55:26Z
dc.date.available
2021-01-22T16:01:55Z
dc.date.available
2021-03-05T14:39:27Z
dc.date.issued
2021-03-04
dc.identifier.issn
1934-5909
dc.identifier.issn
1875-9777
dc.identifier.other
10.1016/j.stem.2020.11.008
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/464395
dc.description.abstract
The human intestinal stem cell niche supports self-renewal and epithelial function, but little is known about its development. We used single-cell mRNA sequencing with in situ validation approaches to interrogate human intestinal development from 7–21 weeks post conception, assigning molecular identities and spatial locations to cells and factors that comprise the niche. Smooth muscle cells of the muscularis mucosa, in close proximity to proliferative crypts, are a source of WNT and RSPONDIN ligands, whereas EGF is expressed far from crypts in the villus epithelium. Instead, an PDGFRAHI/F3HI/DLL1HI mesenchymal population lines the crypt-villus axis and is the source of the epidermal growth factor (EGF) family member NEUREGULIN1 (NRG1). In developing intestine enteroid cultures, NRG1, but not EGF, permitted increased cellular diversity via differentiation of secretory lineages. This work highlights the complexities of intestinal EGF/ERBB signaling and delineates key niche cells and signals of the developing intestine.
en_US
dc.language.iso
en
en_US
dc.publisher
Cell Press
en_US
dc.subject
Intestine
en_US
dc.subject
development
en_US
dc.subject
human
en_US
dc.subject
organoid
en_US
dc.subject
enteroid
en_US
dc.subject
scRNA-seq
en_US
dc.subject
niche
en_US
dc.subject
stem cell
en_US
dc.subject
NRG1
en_US
dc.title
Mapping Development of the Human Intestinal Niche at Single-Cell Resolution
en_US
dc.type
Journal Article
dc.date.published
2020-12-04
ethz.journal.title
Cell Stem Cell
ethz.journal.volume
28
en_US
ethz.journal.issue
3
en_US
ethz.pages.start
568
en_US
ethz.pages.end
580.e4
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Cambridge, MA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::09485 - Treutlein, Barbara / Treutlein, Barbara
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::09485 - Treutlein, Barbara / Treutlein, Barbara
en_US
ethz.date.deposited
2021-01-21T08:55:33Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2021-03-05T14:39:46Z
ethz.rosetta.lastUpdated
2022-03-29T05:38:57Z
ethz.rosetta.versionExported
true
ethz.COinS
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