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dc.contributor.author
Martin, Bryan T.
dc.contributor.author
Malmstrom, Robert D.
dc.contributor.author
Amaro, Rommie E.
dc.contributor.author
Wüthrich, Kurt
dc.date.accessioned
2021-02-05T13:17:04Z
dc.date.available
2021-01-26T06:26:20Z
dc.date.available
2021-02-05T13:17:04Z
dc.date.issued
2021-02-02
dc.identifier.issn
1439-4227
dc.identifier.issn
1439-7633
dc.identifier.other
10.1002/cbic.202000517
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/465485
dc.description.abstract
The 55‐residue OCRE domains of the splicing factors RBM5 and RBM10 contain 15 tyrosines in compact, globular folds. At 25 °C, all 15 tyrosines show symmetric 1H NMR spectra, with averaged signals for the pairs of δ‐ and ϵ‐ring hydrogens. At 4 °C, two tyrosines were identified as showing 1H NMR line‐broadening due to lowered frequency of the ring‐flipping. For the other 13 tyrosine rings, it was not evident, from the 1H NMR data alone, whether they were either all flipping at high frequencies, or whether slowed flipping went undetected due to small chemical‐shift differences between pairs of exchanging ring hydrogen atoms. Here, we integrate 1H NMR spectroscopy and molecular dynamics (MD) simulations to determine the tyrosine ring‐flip frequencies. In the RBM10‐OCRE domain, we found that, for 11 of the 15 tyrosines, these frequencies are in the range 2.0×106 to 1.3×108 s−1, and we established an upper limit of <1.0×106 s−1 for the remaining four residues. The experimental data and the MD simulation are mutually supportive, and their combined use extends the analysis of aromatic ring‐flip events beyond the limitations of routine 1H NMR line‐shape analysis into the nanosecond frequency range. © 2020 Wiley‐VCH GmbH.
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley
en_US
dc.subject
Aromatic ring flips
en_US
dc.subject
Microsecond rate processes
en_US
dc.subject
Nanosecond rate processes
en_US
dc.subject
Protein dynamics
en_US
dc.subject
Tyrosine-rich proteins
en_US
dc.title
OCRE Domains of Splicing Factors RBM5 and RBM10: Tyrosine Ring‐Flip Frequencies Determined by Integrated Use of 1H NMR Spectroscopy and Molecular Dynamics Simulations
en_US
dc.type
Journal Article
dc.date.published
2020-09-25
ethz.journal.title
ChemBioChem
ethz.journal.volume
22
en_US
ethz.journal.issue
3
en_US
ethz.journal.abbreviated
ChemBioChem
ethz.pages.start
565
en_US
ethz.pages.end
570
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Weinheim
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02521 - Inst. f. Molekularbiologie u. Biophysik / Inst. Molecular Biology and Biophysics::03129 - Wüthrich, Kurt / Wüthrich, Kurt
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02521 - Inst. f. Molekularbiologie u. Biophysik / Inst. Molecular Biology and Biophysics::03129 - Wüthrich, Kurt / Wüthrich, Kurt
en_US
ethz.date.deposited
2020-11-17T03:41:59Z
ethz.source
WOS
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2021-02-05T13:17:15Z
ethz.rosetta.lastUpdated
2021-02-05T13:17:15Z
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/451615
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/460170
ethz.COinS
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