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dc.contributor.author
Schoenrath, Felix
dc.contributor.author
Kursawe, Laura
dc.contributor.author
Nersesian, Gaik
dc.contributor.author
Kikhney, Judith
dc.contributor.author
Schmidt, Julia
dc.contributor.author
Barthel, Frank
dc.contributor.author
Kaufmann, Friedrich
dc.contributor.author
Knierim, Jan
dc.contributor.author
Knosalla, Christoph
dc.contributor.author
Hennig, Felix
dc.contributor.author
Falk, Volkmar
dc.contributor.author
Potapov, Evgenij
dc.contributor.author
Moter, Annette
dc.date.accessioned
2021-04-29T10:04:24Z
dc.date.available
2021-01-31T11:32:26Z
dc.date.available
2021-02-26T15:05:15Z
dc.date.available
2021-04-29T10:04:24Z
dc.date.issued
2021-05
dc.identifier.issn
1058-2916
dc.identifier.issn
1538-943X
dc.identifier.other
10.1097/mat.0000000000001260
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/466898
dc.description.abstract
The development of driveline infections following left ventricular assist device (LVAD) implantation remains a major problem. We investigated the impact of fluorescence in situ hybridization (FISH) combined with 16S rRNA gene sequencing on the diagnosis of driveline infections. LVAD drivelines (n = 61) from 60 consecutive patients were obtained during LVAD explantation and subjected to FISH analysis. 16S rRNA gene polymerase chain reaction (PCR) and sequencing to identify the microorganisms were performed. Results were compared with those of a standard microbiological culture. The reasons for pump removal were heart transplantation (n = 22), weaning (n = 14), pump exchange due to pump thrombosis (n = 12), technical problems (n = 7), or death (n = 5). Of the 60 patients, 26 exhibited clinical signs of a VAD-specific infection, while 34 (with 35 drivelines) showed no clinical signs of infection before explantation. The spectrum of identified pathogens differed between FISH/PCR and conventional microbiological diagnostics. In general, the bacterial spectrum was more diverse in FISH/PCR as compared with conventional microbiology, which more often showed only typical skin flora (coagulase-negative staphylococci and Corynebacteriaceae). In addition to identifying the species, FISH/PCR provided information about the spatial distribution and invasiveness of the microorganisms. Cultures usually represent the only source of microbiological information for clinicians and often prove to be unsatisfactory in complex LVAD cases. FISH/PCR not only identified a greater number and variety of microorganisms than standard culture did, but it also provided information about the number, localization, and biofilm state of the pathogens, making it a useful tool for diagnosing the specific cause of LVAD driveline infections.
en_US
dc.language.iso
en
en_US
dc.publisher
Lippincott Williams & Wilkins
en_US
dc.subject
LVAD
en_US
dc.subject
VAD-specific infection
en_US
dc.subject
molecular diagnostics
en_US
dc.subject
FISH
en_US
dc.subject
PCR
en_US
dc.title
Fluorescence In Situ Hybridization and Polymerase Chain Reaction to Detect Infections in Patients With Left Ventricular Assist Devices
en_US
dc.type
Journal Article
dc.date.published
2020-08-18
ethz.journal.title
ASAIO Journal
ethz.journal.volume
67
en_US
ethz.journal.issue
5
en_US
ethz.journal.abbreviated
ASAIO J
ethz.pages.start
536
en_US
ethz.pages.end
545
en_US
ethz.publication.place
Hagerstown, MD
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::09667 - Falk, Volkmar / Falk, Volkmar
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::09667 - Falk, Volkmar / Falk, Volkmar
en_US
ethz.date.deposited
2021-01-31T11:32:40Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2021-04-29T10:04:37Z
ethz.rosetta.lastUpdated
2024-02-02T13:35:32Z
ethz.rosetta.versionExported
true
ethz.COinS
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