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dc.contributor.author
Zhang, Jianhua
dc.contributor.author
Eyisoylu, Hande
dc.contributor.author
Qin, Xiao-Hua
dc.contributor.author
Rubert, Marina
dc.contributor.author
Müller, Ralph
dc.date.accessioned
2021-02-04T15:54:29Z
dc.date.available
2021-02-04T03:57:15Z
dc.date.available
2021-02-04T15:54:29Z
dc.date.issued
2021-02
dc.identifier.issn
1742-7061
dc.identifier.issn
1878-7568
dc.identifier.other
10.1016/j.actbio.2020.12.026
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/467629
dc.identifier.doi
10.3929/ethz-b-000467629
dc.description.abstract
Bioprinting is a promising technique for facilitating the fabrication of engineered bone tissues for patient-specific defect repair and for developing in vitro tissue/organ models for ex vivo tests. However, polymer-based ink materials often result in insufficient mechanical strength, low scaffold fidelity and loss of osteogenesis induction because of the intrinsic swelling/shrinking and bioinert properties of most polymeric hydrogels. Here, we developed a human mesenchymal stem cells (hMSCs)-laden graphene oxide (GO)/alginate/gelatin composite bioink to form 3D bone-mimicking scaffolds using a 3D bioprinting technique. Our results showed that the GO composite bioinks (0.5GO, 1GO, 2GO) with higher GO concentrations (0.5, 1 and 2 mg/ml) improved the bioprintability, scaffold fidelity, compressive modulus and cell viability at day 1. The higher GO concentration increased the cell body size and DNA content, but the 2GO group swelled and had the lowest compressive modulus at day 42. The 1GO group had the highest osteogenic differentiation of hMSC with the upregulation of osteogenic-related gene (ALPL, BGLAP, PHEX) expression. To mimic critical-sized calvarial bone defects in mice and prove scaffold fidelity, 3D cell-laden GO defect scaffolds with complex geometries were successfully bioprinted. 1GO maintained the best scaffold fidelity and had the highest mineral volume after culturing in the bioreactor for 42 days. In conclusion, GO composite bioinks had better bioprintability, scaffold fidelity, cell proliferation, osteogenic differentiation and ECM mineralization than the pure alginate/gelatin system. The optimal GO group was 1GO, which demonstrated the potential for 3D bioprinting of bone tissue models and tissue engineering applications.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
3D bioprinting
en_US
dc.subject
Graphene oxide
en_US
dc.subject
Scaffold fidelity
en_US
dc.subject
Osteogenic differentiation
en_US
dc.subject
ECM mineralization
en_US
dc.title
3D bioprinting of graphene oxide-incorporated cell-laden bone mimicking scaffolds for promoting scaffold fidelity, osteogenic differentiation and mineralization
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2020-12-14
ethz.journal.title
Acta Biomaterialia
ethz.journal.volume
121
en_US
ethz.journal.abbreviated
Acta Biomater
ethz.pages.start
637
en_US
ethz.pages.end
652
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Amsterdam
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02518 - Institut für Biomechanik / Institute for Biomechanics::03565 - Müller, Ralph / Müller, Ralph
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02518 - Institut für Biomechanik / Institute for Biomechanics::03565 - Müller, Ralph / Müller, Ralph
ethz.date.deposited
2021-02-04T03:57:19Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-02-04T15:54:41Z
ethz.rosetta.lastUpdated
2024-02-02T13:02:23Z
ethz.rosetta.versionExported
true
ethz.COinS
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