Show simple item record

dc.contributor.author
Delbrück, Alessia I.
dc.contributor.author
Zhang, Yifan
dc.contributor.author
Hug, Vera
dc.contributor.author
Trunet, Clément
dc.contributor.author
Mathys, Alexander
dc.date.accessioned
2021-03-05T08:11:26Z
dc.date.available
2021-02-12T06:54:14Z
dc.date.available
2021-02-12T14:11:43Z
dc.date.available
2021-03-05T08:11:26Z
dc.date.issued
2021-04-02
dc.identifier.issn
0168-1605
dc.identifier.issn
1879-3460
dc.identifier.other
10.1016/j.ijfoodmicro.2021.109088
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/469150
dc.identifier.doi
10.3929/ethz-b-000469150
dc.description.abstract
Bacterial spores are a major challenge in industrial decontamination processes owing to their extreme resistance. High-pressure (HP) of 150 MPa at 37°C can trigger the germination of spores, making them lose their extreme resistance. Once their resistance is lost, germinated spores can easily be inactivated by a mild decontamination step. The implementation of this gentle germination-inactivation strategy is hindered by the presence of a subpopulation of so-called high-pressure superdormant (HPSD) spores, which resist germination or germinate only very slowly in response to HP. It is essential to understand the properties of HPSD spores and the underlying causes of superdormancy to tackle superdormant spores and further develop germination-inactivation strategies involving HP. This study investigated factors influencing the prevalence of HPSD spores and successfully isolated them by combining buoyant density centrifugation and fluorescence-activated cell sorting, which allowed further characterisation of HPSD spores for the first time. The prevalence of HPSD spores was shown to be strongly dependent on the HP dwell time, with increasing treatment times reducing their prevalence. Spore mutants lacking major germinant receptors further showed a highly increased prevalence of HPSD spores; 93% of the spores remained dormant even after a prolonged HP dwell time of 40 min. In contrast to nutrient germination, sublethal heat treatment of 75°C for 30 min prior to pressure treatment did not induce spore activation and increase germination. The isolated HPSD spores did not show visible structural differences compared to the initial dormant spores when investigated with transmission electron microscopy. Re-sporulated HPSD spores showed similar germination capacity compared to the initial dormant spores, indicating that HPSD spores are most likely not genetically different from the rest of the population. Moreover, the majority of HPSD spores germinated when exposed a second time to the same germination treatment; however, the germination capacity was lower than that of the initial population. The fact that the majority of spores lost superdormancy when exposed a second time to the same trigger makes it unlikely that there is one factor that determines whether a spore germinates with a certain HP treatment or not. Instead, it seems possible that there are other reversible or cumulative causes. This study investigated the factors influencing spore HP superdormancy to improve the understanding of HPSD spores with regard to their stability, germination capacity, and potential underlying causes of spore HP superdormancy. This knowledge will contribute to the development of HP-based germination-inactivation strategies for gentle but effective spore control.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Bacterial spore
en_US
dc.subject
Superdormancy
en_US
dc.subject
Germination
en_US
dc.subject
Heterogeneous
en_US
dc.subject
Flow cytometry
en_US
dc.subject
Mild inactivation
en_US
dc.title
Isolation, stability, and characteristics of high-pressure superdormant Bacillus subtilis spores
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-02-11
ethz.journal.title
International Journal of Food Microbiology
ethz.journal.volume
343
en_US
ethz.journal.abbreviated
Int. j. food microbiol.
ethz.pages.start
109088
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Isolation and characterization of high pressure superdormant spores
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Amsterdam
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::09571 - Mathys, Alexander / Mathys, Alexander
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::09571 - Mathys, Alexander / Mathys, Alexander
en_US
ethz.grant.agreementno
182273
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projekte Lebenswissenschaften
ethz.relation.isSupplementedBy
10.3929/ethz-b-000541446
ethz.date.deposited
2021-02-12T06:54:24Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-03-05T08:11:38Z
ethz.rosetta.lastUpdated
2022-03-29T05:37:57Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Isolation,%20stability,%20and%20characteristics%20of%20high-pressure%20superdormant%20Bacillus%20subtilis%20spores&rft.jtitle=International%20Journal%20of%20Food%20Microbiology&rft.date=2021-04-02&rft.volume=343&rft.spage=109088&rft.issn=0168-1605&1879-3460&rft.au=Delbr%C3%BCck,%20Alessia%20I.&Zhang,%20Yifan&Hug,%20Vera&Trunet,%20Cl%C3%A9ment&Mathys,%20Alexander&rft.genre=article&rft_id=info:doi/10.1016/j.ijfoodmicro.2021.109088&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record