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dc.contributor.author
Dutta, Rahul
dc.contributor.author
Mandal, Subhamoy
dc.contributor.author
Lin, Hsiao-Chun A.
dc.contributor.author
Raz, Tal
dc.contributor.author
Kind, Alexander
dc.contributor.author
Schnieke, Angelika
dc.contributor.author
Razansky, Daniel
dc.date.accessioned
2021-02-19T09:42:07Z
dc.date.available
2021-02-18T04:28:04Z
dc.date.available
2021-02-19T09:42:07Z
dc.date.issued
2020-11-25
dc.identifier.issn
1742-5689
dc.identifier.issn
1742-5662
dc.identifier.other
10.1098/rsif.2020.0776rsif20200776
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/470123
dc.description.abstract
In the field of reproductive biology, there is a strong need for a suitable tool capable of non-destructive evaluation of oocyte viability and function. We studied the application of brilliant cresyl blue (BCB) as an intra-vital exogenous contrast agent using multispectral optoacoustic tomography (MSOT) for visualization of porcine ovarian follicles. The technique provided excellent molecular sensitivity, enabling the selection of competent oocytes without disrupting the follicles. We further conducted in vitro embryo culture, molecular analysis (real-time and reverse transcriptase polymerase chain reaction) and DNA fragmentation analysis to comprehensively establish the safety of BCB-enhanced MSOT imaging in monitoring oocyte viability. Overall, the experimental results suggest that the method offers a significant advance in the use of contrast agents and molecular imaging for reproductive studies. Our technique improves the accurate prediction of ovarian reserve significantly and, once standardized for in vivo imaging, could provide an effective tool for clinical infertility management.
en_US
dc.language.iso
en
en_US
dc.publisher
The Royal Society
en_US
dc.subject
optoacoustic imaging
en_US
dc.subject
Artificial reproductive technology
en_US
dc.subject
medical imaging
en_US
dc.subject
Veterinary medicine
en_US
dc.subject
brilliant cresyl blue
en_US
dc.title
Brilliant cresyl blue enhanced optoacoustic imaging enables non-destructive imaging of mammalian ovarian follicles for artificial reproduction: Brilliant cresyl blue enhanced optoacoustic imaging enables non-destructive imaging of mammalian ovarian follicles for artificial reproduction
en_US
dc.type
Journal Article
dc.date.published
2020-11-04
ethz.journal.title
Journal of the Royal Society. Interface
ethz.journal.volume
17
en_US
ethz.journal.issue
172
en_US
ethz.journal.abbreviated
J. R. Soc. Interface
ethz.pages.start
20200776
en_US
ethz.size
9 p.
en_US
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2021-02-18T04:28:14Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2021-02-19T09:42:18Z
ethz.rosetta.lastUpdated
2021-02-19T09:42:18Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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