Show simple item record

dc.contributor.author
Lakshmanan, Lakshmi N.
dc.contributor.author
Yee, Zhuangli
dc.contributor.author
Halliwell, Barry
dc.contributor.author
Gruber, Jan
dc.contributor.author
Gunawan, Rudiyanto
dc.date.accessioned
2021-03-01T13:28:59Z
dc.date.available
2021-03-01T05:04:48Z
dc.date.available
2021-03-01T13:28:59Z
dc.date.issued
2021-03-19
dc.identifier.issn
2589-0042
dc.identifier.other
10.1016/j.isci.2021.102138
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/472093
dc.identifier.doi
10.3929/ethz-b-000472093
dc.description.abstract
Broad evidence in the literature supports double-strand breaks (DSBs) as initiators of mitochondrial DNA (mtDNA) deletion mutations. While DNA misalignment during DSB repair is commonly proposed as the mechanism by which DSBs cause deletion mutations, details such as the specific DNA repair errors are still lacking. Here, we used DNA hybridization thermodynamics to infer the sequence lengths of mtDNA misalignments that are associated with mtDNA deletions. We gathered and analyzed 9,921 previously reported mtDNA deletion breakpoints in human, rhesus monkey, mouse, rat, and Caenorhabditis elegans. Our analysis shows that a large fraction of mtDNA breakpoint positions can be explained by the thermodynamics of short ≤ 5-nt misalignments. The significance of short DNA misalignments supports an important role for erroneous non-homologous and micro-homology-dependent DSB repair in mtDNA deletion formation. The consistency of the results of our analysis across species further suggests a shared mode of mtDNA deletion mutagenesis.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Cell Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Thermodynamic analysis of DNA hybridization signatures near mitochondrial DNA deletion breakpoints
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
iScience
ethz.journal.volume
24
en_US
ethz.journal.issue
3
en_US
ethz.pages.start
102138
en_US
ethz.size
30 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Ageing, Mitochondria and Mitochondrial DNA
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Cambridge, MA
en_US
ethz.publication.status
published
en_US
ethz.grant.agreementno
150522
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Individuelle Kurzaufenthalte
ethz.date.deposited
2021-03-01T05:04:52Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-03-01T13:29:10Z
ethz.rosetta.lastUpdated
2022-03-29T05:31:05Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Thermodynamic%20analysis%20of%20DNA%20hybridization%20signatures%20near%20mitochondrial%20DNA%20deletion%20breakpoints&rft.jtitle=iScience&rft.date=2021-03-19&rft.volume=24&rft.issue=3&rft.spage=102138&rft.issn=2589-0042&rft.au=Lakshmanan,%20Lakshmi%20N.&Yee,%20Zhuangli&Halliwell,%20Barry&Gruber,%20Jan&Gunawan,%20Rudiyanto&rft.genre=article&rft_id=info:doi/10.1016/j.isci.2021.102138&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record