CRISPRi screens reveal genes modulating yeast growth in lignocellulose hydrolysate
dc.contributor.author
Gutmann, Friederike
dc.contributor.author
Jann, Cosimo
dc.contributor.author
Pereira, Filipa
dc.contributor.author
Johansson, Andreas
dc.contributor.author
Steinmetz, Lars M.
dc.contributor.author
Patil, Kiran R.
dc.date.accessioned
2021-03-01T13:15:49Z
dc.date.available
2021-03-01T05:05:41Z
dc.date.available
2021-03-01T13:15:49Z
dc.date.issued
2021
dc.identifier.issn
1754-6834
dc.identifier.other
10.1186/s13068-021-01880-7
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/472099
dc.identifier.doi
10.3929/ethz-b-000472099
dc.description.abstract
Background
Baker’s yeast is a widely used eukaryotic cell factory, producing a diverse range of compounds including biofuels and fine chemicals. The use of lignocellulose as feedstock offers the opportunity to run these processes in an environmentally sustainable way. However, the required hydrolysis pretreatment of lignocellulosic material releases toxic compounds that hamper yeast growth and consequently productivity.
Results
Here, we employ CRISPR interference in S. cerevisiae to identify genes modulating fermentative growth in plant hydrolysate and in presence of lignocellulosic toxins. We find that at least one-third of hydrolysate-associated gene functions are explained by effects of known toxic compounds, such as the decreased growth of YAP1 or HAA1, or increased growth of DOT6 knock-down strains in hydrolysate.
Conclusion
Our study confirms previously known genetic elements and uncovers new targets towards designing more robust yeast strains for the utilization of lignocellulose hydrolysate as sustainable feedstock, and, more broadly, paves the way for applying CRISPRi screens to improve industrial fermentation processes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Lignocellulose hydrolysate
en_US
dc.subject
Growth-inhibitor compounds
en_US
dc.subject
CRISPR interference screen
en_US
dc.subject
Yeast fermentation
en_US
dc.subject
Sustainable biotechnology
en_US
dc.title
CRISPRi screens reveal genes modulating yeast growth in lignocellulose hydrolysate
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-02-10
ethz.journal.title
Biotechnology for Biofuels
ethz.journal.volume
14
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Biotechnol. biofuels
ethz.pages.start
41
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2021-03-01T05:05:45Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-03-01T13:15:59Z
ethz.rosetta.lastUpdated
2022-03-29T05:30:56Z
ethz.rosetta.versionExported
true
ethz.COinS
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