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dc.contributor.author
Leon-Sampedro, Ricardo
dc.contributor.author
Fernández-de-Bobadilla, Miguel D.
dc.contributor.author
San Millán, Álvaro
dc.contributor.author
Baquero, Fernando
dc.contributor.author
Coque, Teresa M.
dc.date.accessioned
2021-03-09T07:13:16Z
dc.date.available
2021-03-08T15:46:51Z
dc.date.available
2021-03-09T07:13:16Z
dc.date.issued
2019-09
dc.identifier.issn
0305-7453
dc.identifier.issn
1460-2091
dc.identifier.other
10.1093/jac/dkz239
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/473464
dc.description.abstract
Background Tn5801 [tet(M)], a Tn916-like element with site-specific affinity for the 3′ end of the housekeeping gene guaA, may integrate at different chromosomal sites. Objectives To characterize the genetic context of Tn5801 to define its transfer dynamics and impact on the evolution of Enterococcus faecalis (Efs). Methods WGS (Illumina HiSeq 2500) was performed on the Efs clinical strain Ef1 and primary and secondary transconjugants of Efs strains JH2-2 [which naturally contains Tn5801.B23, an unusual variant that lacks tet(M)], OG1RF and OG1SS carrying different copies of Tn5801-like elements. The transposon structures were analysed using a range of bioinformatics tools allowing us to identify the context of Tn5801-like elements. Growth rates at different tetracycline concentrations (0.5–20 mg/L) were estimated using a Synergy HTX plate reader. Results Tn5801.B15 [tet(M), 20.3 kb] exists and can be transferred either singly or within Tn6648 (53.2 kb), a composite element that comprises Tn5801.B15 and Tn6647, a newly identified 32.8 kb transposon that contains the prgABCT operon of pheromone-responsive plasmids. These transposons are able to integrate at specific 11 nt sequences at the 3′ end of guaA and at other chromosomal sites in Efs genomes, thus being able to generate tandem accretions. These events may increase the number of tet(M) copies, enhancing tetracycline resistance in the recipient strain. Conclusions This study describes Tn6647 and Tn6648 (comprising Tn6647 and Tn5801.B15) and highlights the diversity of mechanisms for conjugative mobilization and chromosomal insertion of these elements, which can result in tandem accretion. This strategy would facilitate the adaptation of Efs clones to environmental challenges.
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.title
Transfer dynamics of Tn6648, a composite integrative conjugative element generated by tandem accretion of Tn5801 and Tn6647 in Enterococcus faecalis
en_US
dc.type
Journal Article
dc.type
Journal Article
dc.date.published
2019-06-21
ethz.journal.title
Journal of Antimicrobial Chemotherapy
ethz.journal.volume
74
en_US
ethz.journal.issue
9
en_US
ethz.journal.abbreviated
J Antimicrob Chemother
ethz.pages.start
2517
en_US
ethz.pages.end
2523
en_US
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::09497 - Hall, Alex / Hall, Alex
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::09497 - Hall, Alex / Hall, Alex
en_US
ethz.identifier.orcidWorkCode
58722216
ethz.date.deposited
2021-03-08T15:47:01Z
ethz.source
FORM
ethz.eth
no
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2021-03-09T07:13:39Z
ethz.rosetta.lastUpdated
2022-03-29T05:40:20Z
ethz.rosetta.versionExported
true
ethz.COinS
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