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dc.contributor.author
Leon-Sampedro, Ricardo
dc.contributor.author
Novais, Carla
dc.contributor.author
Peixe, Luísa
dc.contributor.author
Baquero, Fernando
dc.contributor.author
Coque, Teresa M.
dc.date.accessioned
2021-03-09T06:32:50Z
dc.date.available
2021-03-08T15:47:41Z
dc.date.available
2021-03-09T06:32:50Z
dc.date.issued
2016-03
dc.identifier.issn
0066-4804
dc.identifier.issn
1098-6596
dc.identifier.other
10.1128/AAC.01864-15
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/473465
dc.description.abstract
This work describes the diversity and evolution of Tn5801 among enterococci, staphylococci, and streptococci based on analysis of the 5,073 genomes of these bacterial groups available in gene databases. We also examined 610 isolates of Enterococcus (from 10 countries, 1987 to 2010) for the presence of this and other known CTn-tet(M) elements due to the scarcity of data about Tn5801 among enterococci. Genome location (by ICeu-I–pulsed-field gel electrophoresis [PFGE] hybridization/integration site identification), conjugation and fitness (by standard methods), Tn5801 characterization (by long-PCR mapping/sequencing), and clonality (by PFGE/multilocus sequence typing [MLST]) were studied. Twenty-three Tn5801 variants (17 unpublished) clustered in two groups, designated “A” (25 kb; n = 14; predominant in Staphylococcus aureus) and “B” (20 kb; n = 9; predominant in Streptococcus agalactiae). The percent GC content of the common backbone suggests a streptococcal origin of Tn5801 group B, with further acquisition of a 5-kb fragment that resulted in group A. Deep sequence analysis allowed identification of variants associated with clonal lineages of S. aureus (clonal complex 8 [CC8], sequence type 239 [ST239]), S. agalactiae (CC17), Enterococcus faecium (ST17/ST18), or Enterococcus faecalis (ST8), local variants, or variants located in different species and geographical areas. All Tn5801 elements were chromosomally located upstream of the guaA gene, which serves as an integration hot spot. Transferability was demonstrated only for Tn5801 type B among E. faecalis clonal backgrounds, which eventually harbored another Tn5801 copy. The study documents early acquisition of Tn5801 by Enterococcus, Staphylococcus, and Streptococcus. Clonal waves of these pathogens seem to have contributed to the geographical spread and local evolution of the transposon. Horizontal transfer, also demonstrated, could explain the variability observed, with the isolates often containing sequences of different origins.
en_US
dc.language.iso
en
en_US
dc.publisher
American Society for Microbiology
en_US
dc.title
Diversity and evolution of the Tn5801-tet(M)-like integrative and conjugative elements among Enterococcus, streptococcus, and Staphylococcus
en_US
dc.type
Journal Article
dc.type
Journal Article
dc.date.published
2016-02-26
ethz.journal.title
Antimicrobial Agents and Chemotherapy
ethz.journal.volume
60
en_US
ethz.journal.issue
3
en_US
ethz.journal.abbreviated
Antimicrob Agents Chemother
ethz.pages.start
1736
en_US
ethz.pages.end
1746
en_US
ethz.identifier.scopus
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::09497 - Hall, Alex / Hall, Alex
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::09497 - Hall, Alex / Hall, Alex
en_US
ethz.identifier.orcidWorkCode
35435296
ethz.date.deposited
2021-03-08T15:47:51Z
ethz.source
FORM
ethz.eth
no
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2021-03-09T06:33:01Z
ethz.rosetta.lastUpdated
2022-03-29T05:40:18Z
ethz.rosetta.versionExported
true
ethz.COinS
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