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dc.contributor.author
Curiao, Tânia
dc.contributor.author
Marchi, Emmanuela
dc.contributor.author
Grandgirard, Denis
dc.contributor.author
Leon-Sampedro, Ricardo
dc.contributor.author
Viti, Carlo
dc.contributor.author
Leib, Stephen L.
dc.contributor.author
Baquero, Fernando
dc.contributor.author
Oggioni, Marco R.
dc.contributor.author
Martinez, José Luis
dc.contributor.author
Coque, Teresa M.
dc.date.accessioned
2021-03-11T15:23:31Z
dc.date.available
2021-03-08T15:52:05Z
dc.date.available
2021-03-11T15:23:31Z
dc.date.issued
2016
dc.identifier.other
10.1186/s12864-016-2778-z
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/473467
dc.identifier.doi
10.3929/ethz-b-000473467
dc.description.abstract
Background Biocides and antibiotics are used to eradicate or prevent the growth of microbial species on surfaces (occasionally on catheters), or infected sites, either in combination or sequentially, raising concerns about the development of co-resistance to both antimicrobial types. The effect of such compounds on Salmonella enterica, a major food-borne and zoonotic pathogen, has been analysed in different studies, but only few works evaluated its biological cost, and the overall effects at the genomic and transcriptomic levels associated with diverse phenotypes resulting from biocide exposure, which was the aim of this work. Results Exposure to triclosan, clorhexidine, benzalkonium, (but not to hypochlorite) resulted in mutants with different phenotypes to a wide range of antimicrobials even unrelated to the selective agent. Most biocide-resistant mutants showed increased susceptibility to compounds acting on the cell wall (β-lactams) or the cell membranes (poly-L-lysine, polymyxin B, colistin or toxic anions). Mutations (SNPs) were found in three intergenic regions and nine genes, which have a role in energy production, amino acids, carbohydrates or lipids metabolism, some of them involved in membrane transport and pathogenicity. Comparative transcriptomics of biocide-resistant mutants showed over-expression of genes encoding efflux pumps (sugE), ribosomal and transcription-related proteins, cold-shock response (cpeE) and enzymes of microaerobic metabolism including those of the phosphotransferase system. Mainly ribosomal, metabolic and pathogenicity-related genes had affected expression in both in vitro-selected biocide mutants and field Salmonella isolates with reduced biocide susceptibility. Conclusions Multiple pathways can be involved in the adaptation of Salmonella to biocides, mainly related with global stress, or involving metabolic and membrane alterations, and eventually causing “collateral sensitivity” to other antimicrobials. These changes might impact the bacterial-environment interaction, imposing significant bacterial fitness costs which may reduce the chances of fixation and spread of biocide resistant mutants.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Biocide resistance
en_US
dc.subject
Antimicrobial susceptibility
en_US
dc.subject
WGS
en_US
dc.subject
Transcriptomics
en_US
dc.subject
Collateral sensitivity
en_US
dc.title
Multiple adaptive routes of Salmonella enterica Typhimurium to biocide and antibiotic exposure
en_US
dc.type
Journal Article
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2016-07-13
ethz.journal.title
BMC Genomics
ethz.journal.volume
17
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
491
en_US
ethz.size
16 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::09497 - Hall, Alex / Hall, Alex
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::09497 - Hall, Alex / Hall, Alex
en_US
ethz.identifier.orcidWorkCode
35435294
ethz.date.deposited
2021-03-08T15:52:15Z
ethz.source
FORM
ethz.eth
no
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-03-11T15:23:42Z
ethz.rosetta.lastUpdated
2022-03-29T05:43:55Z
ethz.rosetta.versionExported
true
ethz.COinS
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