Methods to Quantify DNA Transfer in Enterococcus
dc.contributor.author
Novais, Carla
dc.contributor.author
Freitas, Ana R.
dc.contributor.author
Leon-Sampedro, Ricardo
dc.contributor.author
Peixe, Luísa
dc.contributor.author
Coque, Teresa M.
dc.contributor.editor
de la Cruz, Fernando
dc.date.accessioned
2021-03-10T11:02:52Z
dc.date.available
2021-03-08T15:53:34Z
dc.date.available
2021-03-10T11:02:52Z
dc.date.issued
2020
dc.identifier.isbn
978-1-4939-9876-0
en_US
dc.identifier.isbn
978-1-4939-9877-7
en_US
dc.identifier.issn
1064-3745
dc.identifier.issn
1940-6029
dc.identifier.other
10.1007/978-1-4939-9877-7_8
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/473468
dc.description.abstract
DNA uptake in Enterococcus normally occurs by conjugation, a natural process that is replicated in biomedical research to assess the transferability of different mobile genetic elements and chromosomal regions as well as to study the host range of plasmids and other conjugative elements. More efficient artificial methods to transform cells with foreign DNA as chemotransformation and electroporation are widely used in molecular genetics. Here, we described conjugation protocols to quantify DNA transfer among Enterococcus and revise current perspectives and lab strains. Protocols of electrotransformation have been previously described in this series.
en_US
dc.language.iso
en
en_US
dc.publisher
Humana
en_US
dc.subject
Conjugation
en_US
dc.subject
Plasmids
en_US
dc.subject
Conjugative transposons (CTn)
en_US
dc.subject
Integrative conjugative elements (ICEs)
en_US
dc.subject
Horizontal gene transfer (HGT)
en_US
dc.subject
Enterococcus
en_US
dc.title
Methods to Quantify DNA Transfer in Enterococcus
en_US
dc.type
Book Chapter
dc.date.published
2019-10-05
ethz.book.title
Horizontal Gene Transfer
en_US
ethz.journal.title
Methods in Molecular Biology
ethz.journal.volume
2075
en_US
ethz.journal.abbreviated
Methods Mol Biol
ethz.pages.start
111
en_US
ethz.pages.end
122
en_US
ethz.notes
Protocol
en_US
ethz.identifier.pubmed
31584158
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::09497 - Hall, Alex / Hall, Alex
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::09497 - Hall, Alex / Hall, Alex
en_US
ethz.identifier.orcidWorkCode
88515643
ethz.date.deposited
2021-03-08T15:53:44Z
ethz.source
FORM
ethz.eth
no
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2021-03-10T11:03:04Z
ethz.rosetta.lastUpdated
2022-03-29T05:41:29Z
ethz.rosetta.versionExported
true
ethz.COinS
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Publikationstyp
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Book Chapter [9058]