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dc.contributor.author
Freitas, Ana R.
dc.contributor.author
Elghaieb, Houyem
dc.contributor.author
Leon Sampedro, Ricardo
dc.contributor.author
Abbassi, Mohamed Salah
dc.contributor.author
Novais, Carla
dc.contributor.author
Coque, Teresa M.
dc.contributor.author
Hassen, Abdennaceur
dc.contributor.author
Peixe, Luísa
dc.date.accessioned
2021-03-11T15:40:58Z
dc.date.available
2021-03-08T16:04:54Z
dc.date.available
2021-03-11T15:40:58Z
dc.date.issued
2017-12
dc.identifier.issn
0305-7453
dc.identifier.issn
1460-2091
dc.identifier.other
10.1093/jac/dkx321
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/473478
dc.description.abstract
Objectives Oxazolidinone resistance is a serious limitation in the treatment of MDR Enterococcus infections. Plasmid-mediated oxazolidinone resistance has been strongly linked to animals where the use of phenicols might co-select resistance to both antibiotic families. Our goal was to assess the diversity of genes conferring phenicol/oxazolidinone resistance among diverse enterococci and to characterize the optrA genetic environment. Methods Chloramphenicol-resistant isolates (>16 mg/L, n = 245) from different sources (hospitals/healthy humans/wastewaters/animals) in Portugal, Angola and Tunisia (1996–2016) were selected. Phenicol (eight cat variants, fexA, fexB) or phenicol + oxazolidinone [cfr, cfr(B), optrA] resistance genes were searched for by PCR. Susceptibility (disc diffusion/microdilution), filter mating, stability of antibiotic resistance (500 bacterial generations), plasmid typing (S1-PFGE/hybridization), MLST and WGS (Illumina-HiSeq) were performed for optrA-positive isolates. Results Resistance to phenicols (n = 181, 74%) and phenicols + oxazolidinones (n = 2, 1%) was associated with the presence of cat(A-8) (40%, predominant in hospitals and swine), cat(A-7) (29%, predominant in poultry and healthy humans), cat(A-9) (2%), fexB (2%) and fexA + optrA (1%). fexA and optrA genes were co-located in a transferable plasmid (pAF379, 72 918 bp) of two ST86 MDR Tunisian Enterococcus faecalis (wastewaters) carrying several putative virulence genes. MICs of chloramphenicol, linezolid and tedizolid were stably maintained at 64, 4 and 1 mg/L, respectively. The chimeric pAF379 comprised relics of genetic elements from different Gram-positive bacteria and origins (human/porcine). Conclusions To the best of our knowledge, we report the first detection of optrA in an African country (Tunisia) within a transferable mosaic plasmid of different origins. Its identification in isolates from environmental sources is worrisome and alerts for the need of a concerted global surveillance on the occurrence and spread of optrA.
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.title
Detection of optrA in the African continent (Tunisia) within a mosaic Enterococcus faecalis plasmid from urban wastewaters
en_US
dc.type
Journal Article
dc.type
Journal Article
dc.date.published
2017-09-26
ethz.journal.title
The Journal of Antimicrobial Chemotherapy
ethz.journal.volume
72
en_US
ethz.journal.issue
12
en_US
ethz.journal.abbreviated
J Antimicrob Chemother
ethz.pages.start
3245
en_US
ethz.pages.end
3251
en_US
ethz.identifier.scopus
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::09497 - Hall, Alex / Hall, Alex
en_US
ethz.identifier.orcidWorkCode
42263591
ethz.date.deposited
2021-03-08T16:05:04Z
ethz.source
FORM
ethz.eth
no
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2021-03-11T15:41:09Z
ethz.rosetta.lastUpdated
2021-03-11T15:41:09Z
ethz.rosetta.versionExported
true
ethz.COinS
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