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dc.contributor.author
Brown, Phillip
dc.contributor.author
Chen, Yi
dc.contributor.author
Parsons, Cameron
dc.contributor.author
Brown, Eric
dc.contributor.author
Loessner, Martin J.
dc.contributor.author
Shen, Yang
dc.contributor.author
Kathariou, Sophia
dc.date.accessioned
2021-03-17T10:18:02Z
dc.date.available
2021-03-14T05:21:28Z
dc.date.available
2021-03-17T10:18:02Z
dc.date.issued
2021-02
dc.identifier.issn
2076-0817
dc.identifier.other
10.3390/pathogens10020199
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/474305
dc.identifier.doi
10.3929/ethz-b-000474305
dc.description.abstract
Listeria monocytogenes is a Gram-positive bacterial pathogen and the causative agent of listeriosis, a severe foodborne infection. L. monocytogenes is notorious for its ability to persist in food processing environments (FPEs) via a variety of adaptive traits. Even though traits such as cold tolerance, biofilm formation and sanitizer resistance have been extensively investigated for their roles in persistence of L. monocytogenes in FPEs, much less is known about resistance to bacteriophages. Previous studies explored phage resistance mechanisms in laboratory-created mutants but it is imperative to investigate phage resistance that is naturally exhibited in FPE-derived strains. Here, we integrated the analysis of whole genome sequence data from a panel of serotype 1/2a strains of sequence types 321 and 391 from turkey processing plants, with the determination of cell surface substituents required for phage adsorption and phage infection assays with the four wide-host-range phages A511, P100, 20422-1 and 805405-1. Using a specific set of recombinant phage protein probes, we discovered that phage-resistant strains lacked one or both of the serogroup 1/2-specific wall teichoic acid carbohydrate decorations, N-acetylglucosamine and rhamnose. Furthermore, these phage-resistant strains harbored substitutions in lmo1080, lmo1081, and lmo2550, which mediate carbohydrate decoration of the wall teichoic acids.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
MDPI
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Listeria
en_US
dc.subject
bacteriophage
en_US
dc.subject
phage resistance
en_US
dc.subject
whole genome sequencing
en_US
dc.subject
food processing plant
en_US
dc.subject
InlA
en_US
dc.subject
serotype 1/2a
en_US
dc.subject
wall teichoic acid
en_US
dc.title
Whole genome sequence analysis of phage-resistant listeria monocytogenes serotype 1/2a strains from turkey processing plants
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-02-13
ethz.journal.title
Pathogens
ethz.journal.volume
10
en_US
ethz.journal.issue
2
en_US
ethz.journal.abbreviated
Pathogens
ethz.pages.start
199
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Basel
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03651 - Loessner, Martin / Loessner, Martin
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03651 - Loessner, Martin / Loessner, Martin
ethz.date.deposited
2021-03-14T05:21:42Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-03-17T10:18:17Z
ethz.rosetta.lastUpdated
2022-03-29T05:49:50Z
ethz.rosetta.versionExported
true
ethz.COinS
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